Kotandeniya Delshanee, Carmella Steven G, Ming Xun, Murphy Sharon E, Hecht Stephen S
Masonic Cancer Center, University of Minnesota , 2231 6th Street SE, 2-148 CCRB, Minneapolis, Minnesota 55455, United States.
Anal Chem. 2015 Feb 3;87(3):1514-7. doi: 10.1021/ac504047j. Epub 2015 Jan 14.
Two of the most widely measured compounds in the urine of people who use tobacco products are cotinine, a major metabolite of the addictive constituent nicotine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a metabolite of the powerful lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Thousands of analyses have been reported in the literature, carried out exclusively, to the best of our knowledge, by separate methods. In the study reported here, we have developed a sensitive, accurate, and precise liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring method for the combined analysis of total cotinine (the sum of cotinine and its glucuronide) and total NNAL (the sum of NNAL and its glucuronide). The new method quantifies naturally occurring [(13)C]cotinine to minimize problems associated with the vast differences in concentration of total cotinine and total NNAL in urine. This method should greatly facilitate future determinations of these important compounds.
在使用烟草制品的人群尿液中，检测最为广泛的两种化合物是可替宁（成瘾成分尼古丁的主要代谢产物）和4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁醇（NNAL，强效肺癌致癌物4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁酮（NNK）的代谢产物）。据我们所知，文献中已报道了数千次分析，这些分析均采用单独的方法进行。在本文报道的研究中，我们开发了一种灵敏、准确且精密的液相色谱-电喷雾电离-串联质谱-选择反应监测方法，用于联合分析总可替宁（可替宁及其葡萄糖醛酸苷的总和）和总NNAL（NNAL及其葡萄糖醛酸苷的总和）。新方法对天然存在的[¹³C]可替宁进行定量，以尽量减少因尿液中总可替宁和总NNAL浓度差异巨大而产生的问题。该方法应能极大地促进未来对这些重要化合物的测定工作。
