Laboratório de Tecnologia de Alimentos, CCTA, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes 28013-602, Brazil.
Laboratório de Clínica e Cirurgia Animal, CCTA, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes 28013-602, Brazil.
Molecules. 2017 Oct 23;22(10):1792. doi: 10.3390/molecules22101792.
This study identified two phenolic compounds in Raddi fruits: naringenin (first report in this species) and gallic acid. Their structures were elucidated by nuclear magnetic resonance (NMR) data (¹H-, C-NMR) and a high-performance liquid chromatography (HPLC) technique. A high content of phenolics (659.21 mg of gallic acid equivalents/g of sample-Folin-Ciocalteau method) and total flavonoids (140.69 mg of rutin equivalents/g of sample-aluminum chloride method) were quantified in , as well as high antioxidant activity (77.47%-2,2-diphenyl-1-picrylhydrazyl, DPPH method). The antihypertensive activity related to its phenolic content was investigated. After intravenous infusion in Wistar rats, these phenolics significantly reduced ( < 0.05) the systolic, median, and diastolic arterial pressures of individuals. The rotarod test was performed to determine the mechanism of action of the sample vasorelaxant effect. It was found that its action exceeded that of the positive control used (diazepam). This confirmed the vasodilatory activity exerted by fruits is related to the phenolic compounds present in the plant, which are potent antioxidants and inhibit oxidative stress, mainly in the central nervous system.
柚皮苷（该物种的首次报道）和没食子酸。其结构通过核磁共振（NMR）数据（¹H-，C-NMR）和高效液相色谱（HPLC）技术阐明。采用 Folin-Ciocalteau 法测定，枳椇果实中酚类化合物含量（659.21mg 没食子酸当量/g 样品）和总黄酮（140.69mg 芦丁当量/g 样品-氯化铝法）含量较高，抗氧化活性也较高（77.47%-2,2-二苯基-1-苦基肼基，DPPH 法）。研究了与酚类含量相关的降压活性。静脉输注给 Wistar 大鼠后，这些酚类物质显著降低了（<0.05）个体的收缩压、中值和舒张压。进行了旋转棒试验以确定样品血管舒张作用的作用机制。结果发现，其作用超过了使用的阳性对照（地西泮）。这证实了枳椇果实的血管舒张活性与植物中存在的酚类化合物有关，这些化合物是有效的抗氧化剂，可抑制氧化应激，主要在中枢神经系统中。
