Machu Ludmila, Misurcova Ladislava, Ambrozova Jarmila Vavra, Orsavova Jana, Mlcek Jiri, Sochor Jiri, Jurikova Tunde
Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlin, nam. T. G. Masaryka 5555, Zlin CZ-760 01, Czech Republic.
Language Centre, Faculty of Humanities, Tomas Bata University in Zlin, Mostní 5139, Zlin CZ-760 01, Czech Republic.
Molecules. 2015 Jan 12;20(1):1118-33. doi: 10.3390/molecules20011118.
The study objective was to investigate total phenolic content using Folin-Ciocalteu's method, to assess nine phenols by HPLC, to determine antioxidant capacity of the water soluble compounds (ACW) by a photochemiluminescence method, and to calculate the correlation coefficients in commercial algal food products from brown (Laminaria japonica, Eisenia bicyclis, Hizikia fusiformis, Undaria pinnatifida) and red (Porphyra tenera, Palmaria palmata) seaweed, green freshwater algae (Chlorella pyrenoidosa), and cyanobacteria (Spirulina platensis). HPLC analysis showed that the most abundant phenolic compound was epicatechin. From spectrophotometry and ACW determination it was evident that brown seaweed Eisenia bicyclis was the sample with the highest phenolic and ACW values (193 mg·g-1 GAE; 7.53 µmol AA·g-1, respectively). A linear relationship existed between ACW and phenolic contents (r = 0.99). Some algal products seem to be promising functional foods rich in polyphenols.
该研究的目的是采用福林-西奥尔特法测定总酚含量，通过高效液相色谱法评估9种酚类物质，用光化学发光法测定水溶性化合物的抗氧化能力（ACW），并计算来自褐藻（海带、裙带菜、羊栖菜、裙带菜）、红藻（条斑紫菜、掌状红皮藻）、绿藻（蛋白核小球藻）和蓝细菌（钝顶螺旋藻）的商业藻类食品中的相关系数。高效液相色谱分析表明，最丰富的酚类化合物是表儿茶素。从分光光度法和ACW测定结果来看，明显褐藻裙带菜是酚类和ACW值最高的样品（分别为193 mg·g-1 GAE；7.53 µmol AA·g-1）。ACW与酚类含量之间存在线性关系（r = 0.99）。一些藻类产品似乎是富含多酚的有前景的功能性食品。
