Salehi Sahar, Fathi Mohammadhossein, Javanmard Shaghayegh Haghjooy, Barneh Farnaz, Moshayedi Mona
Department of Materials Engineering, Biomaterials Research Group, Isfahan University of Technology, Isfahan, Iran.
Department of Physiology, Applied, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2015 Jan 6;4:9. doi: 10.4103/2277-9175.148291. eCollection 2015.
Biodegradable elastomeric materials such as poly glycerol sebacate (PGS) have gained much current attention in the field of soft tissue engineering. The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial (HCE) cells viability in vitro.
PGS films were prepared by the casting method. We tried to fabricate PGS with different compositions and various properties as being a viable alternative to the corneal stroma in cornea tissue engineering. The chemical properties of the prepared polymer were investigated by means of attenuated total reflectance - Fourier transform infrared spectroscopy (ATR-FTIR) analysis and the in vitro cytotoxicity was investigated by the Alamarblue method.
The functional groups observed in the PGS FTIR spectrums of PGS with various molar ratios were the same. However, the main difference was the time of completing the cross-linking reaction. The PGS prepared by 2:3 ratio as a molar ratio had the fastest and the 3:2 ratio had the lowest cross-linking rate because of the higher amount of sebacic acid. Results of the Alamarblue cytotoxicity test assay showed no deleterious effect on HCE cell viability and proliferation.
PGS is a potentially good candidate material for corneal tissue engineering because of its lack of in vitro HCE cell toxicity.
可生物降解的弹性体材料，如聚癸二酸甘油酯（PGS），目前在软组织工程领域备受关注。本研究报道了通过缩聚反应合成甘油与癸二酸摩尔比为1:1、2:3和3:2的PGS，并测试了PGS对人角膜上皮（HCE）细胞体外活力的影响。
采用流延法制备PGS薄膜。我们试图制备具有不同组成和各种性能的PGS，作为角膜组织工程中角膜基质的可行替代物。通过衰减全反射-傅里叶变换红外光谱（ATR-FTIR）分析研究制备的聚合物的化学性质，并通过Alamarblue法研究体外细胞毒性。
不同摩尔比的PGS的PGS傅里叶变换红外光谱中观察到的官能团相同。然而，主要差异在于交联反应完成的时间。由于癸二酸含量较高，以2:3摩尔比制备的PGS交联速度最快，3:2摩尔比的交联速度最低。Alamarblue细胞毒性试验结果表明，对HCE细胞活力和增殖没有有害影响。
由于PGS对HCE细胞无体外毒性，它是角膜组织工程中一种潜在的良好候选材料。
