用于检测单峰骆驼中东呼吸综合征冠状病毒抗原的快速免疫层析检测法的开发与验证
Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
作者信息
Song Daesub, Ha Gunwoo, Serhan Wissam, Eltahir Yassir, Yusof Mohammed, Hashem Farouq, Elsayed Elsaeid, Marzoug Bahaaeldin, Abdelazim Assem, Al Muhairi Salama
机构信息
Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Yuseong-gu, Daejeon, South Korea.
BioNote, Inc., Hwaseong-si, Gyeonggi-do, South Korea.
出版信息
J Clin Microbiol. 2015 Apr;53(4):1178-82. doi: 10.1128/JCM.03096-14. Epub 2015 Jan 28.
Abstract
We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
摘要
我们在此展示一种快速免疫层析检测法，用于检测单峰骆驼鼻拭子中的中东呼吸综合征冠状病毒（MERS-CoV）抗原。该检测法基于在室温下使用高选择性单克隆抗体在短时间内检测MERS-CoV核衣壳蛋白。与UpE和开放阅读框1A（Orf1A）实时逆转录聚合酶链反应（RT-PCR）相比，该检测法的相对灵敏度和特异性分别为93.90%和100%。结果表明，此处开发的检测法是用于快速诊断和流行病学监测单峰骆驼中MERS-CoV感染的有用工具。
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Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP).利用逆转录环介导等温扩增技术（RT-LAMP）检测中东呼吸综合征冠状病毒
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