Özcengiz Gülay, Öğülür İsmail
Department of Biological Sciences and Molecular Biology and Genetics, Middle East Technical University, 06800 Ankara, Turkey.
Marmara University, Research and Training Hospital, Division of Pediatric Allergy and Immunology, 34899 Istanbul, Turkey.
N Biotechnol. 2015 Dec 25;32(6):612-9. doi: 10.1016/j.nbt.2015.01.006. Epub 2015 Jan 30.
Bacillus subtilis has the capacity to produce more than two dozen bioactive compounds with an amazing variety of chemical structures. Among them, bacilysin is a non-ribosomally synthesized dipeptide antibiotic consisting of l-alanine residue at the N terminus and a non-proteinogenic amino acid, l-anticapsin, at the C terminus. In spite of its simple structure, it is active against a wide range of bacteria and fungi. As a potent antimicrobial agent, we briefly review the biochemistry and genetics as well as the regulation of bacilysin biosynthesis within the frame of peptide pheromones-based control of secondary activities. Biological functions of bacilysin in the producer B. subtilis beyond its antimicrobial activity as well as potential biotechnological use of the biosynthetic enzyme l-amino acid ligase (Lal) are also discussed.
枯草芽孢杆菌能够产生二十多种具有惊人化学结构多样性的生物活性化合物。其中，杆菌溶素是一种非核糖体合成的二肽抗生素，其N端由L-丙氨酸残基组成，C端由一种非蛋白质氨基酸L-抗荚膜菌素组成。尽管其结构简单，但它对多种细菌和真菌都有活性。作为一种有效的抗菌剂，我们在基于肽信息素控制次级活性的框架内，简要回顾了杆菌溶素生物合成的生物化学、遗传学以及调控。还讨论了杆菌溶素在产生菌枯草芽孢杆菌中除抗菌活性之外的生物学功能，以及生物合成酶L-氨基酸连接酶（Lal）的潜在生物技术用途。
