Hematology and Blood Transfusion Center, University of Campinas, Rua Carlos Chagas, 480, P.O. Box: 6198, Campinas, SP, 13083-878, Brazil,
Support Care Cancer. 2015 Sep;23(9):2561-7. doi: 10.1007/s00520-015-2613-0. Epub 2015 Feb 5.
Recent studies have considered the qualitative and quantitative assessment of salivary flow, as well the biochemical components of saliva, as possible biomarkers that might contribute to the pathogenesis of chronic graft-versus-host disease (cGHVD) in hematopoietic stem cell transplantation (HSCT) patients. The aim of this study was to evaluate prospectively the inorganic salivary status at different periods of allogeneic HSCT. Saliva collection and oral examination were performed prior to the HSCT, between days 8 and 10, days 80 and 100, and at the cGVHD onset. Concentrations of calcium (Ca), phosphate (Pi), chloride (Cl), magnesium (Mg), potassium (K), and sodium (Na) were performed using colorimetric reactions and atomic absorption. Fifty-five consecutive patients undergoing first allogeneic HSCT were included in this study. Between days 8 and 10, the salivary flow rate was significantly higher (p = 0.05), Pi concentration was decreased (p = 0.007), and Na and Cl were increased (p = 0.001 and p = 0.001, respectively), compared with the baseline. Salivary flow rate during the same period showed a negative correlation with Pi concentration (p = 0.02) and a positive correlation with Na and Cl concentrations (p = 0.003 and p = 0.001, respectively). The salivary flow rate was decreased between days 80 and 100 (p = 0.02) and Na, Cl, and K concentrations were increased (p = 0.03, p = 0.02, and p = 0.003, respectively). Salivary flow rate showed a negative correlation with Na and Cl (p = 0.01 and p = 0.013, respectively). At cGVHD onset, the salivary flow rate showed no statistical difference compared with the other studied periods. A trend was observed in the higher Na concentration compared with the baseline (p = 0.06) and Pi concentration presented a significant decrease (p = 0.004). Ca and Mg concentrations showed no changes during all evaluation periods. The present study showed changes in inorganic salivary components in post-HSCT periods, mainly during the early period post-HSCT and at the cGVHD onset. We speculate that Na, Cl, and Pi in saliva could be used as a potential biomarker in further studies.
最近的研究已经考虑了唾液流量的定性和定量评估，以及唾液的生化成分，作为可能有助于造血干细胞移植（HSCT）患者慢性移植物抗宿主病（cGVHD）发病机制的生物标志物。本研究的目的是前瞻性评估同种异体 HSCT 不同时期的唾液无机状态。在 HSCT 之前、第 8-10 天、第 80-100 天和 cGVHD 发病时采集唾液并进行口腔检查。使用比色反应和原子吸收法测定钙（Ca）、磷酸盐（Pi）、氯（Cl）、镁（Mg）、钾（K）和钠（Na）的浓度。55 例连续接受首次同种异体 HSCT 的患者纳入本研究。第 8-10 天时，唾液流速明显升高（p=0.05），Pi 浓度降低（p=0.007），Na 和 Cl 浓度升高（p=0.001 和 p=0.001），与基线相比。同期唾液流速与 Pi 浓度呈负相关（p=0.02），与 Na 和 Cl 浓度呈正相关（p=0.003 和 p=0.001）。第 80-100 天唾液流速降低（p=0.02），Na、Cl 和 K 浓度升高（p=0.03、p=0.02 和 p=0.003）。唾液流速与 Na 和 Cl 呈负相关（p=0.01 和 p=0.013）。cGVHD 发病时，与其他研究期相比，唾液流速无统计学差异。与基线相比，Na 浓度呈升高趋势（p=0.06），Pi 浓度显著降低（p=0.004）。Ca 和 Mg 浓度在所有评估期间均无变化。本研究显示 HSCT 后时期唾液无机成分发生变化，主要发生在 HSCT 后早期和 cGVHD 发病时。我们推测唾液中的 Na、Cl 和 Pi 可以作为进一步研究的潜在生物标志物。
