Fernandez E, Pallini R, Gangitano C, Del Fá A, Sangiacomo C O, Sbriccoli A, Ricoy J R, Rossi G F
Institute of Neurosurgery, Catholic University School of Medicine, Rome, Italy.
Neurol Res. 1989 Mar;11(1):57-62. doi: 10.1080/01616412.1989.11739863.
Three pharmacological agents, L-carnitine, L-acetylcarnitine and gangliosides, were tested for their ability to enhance the regeneration of the rat sciatic nerve following transection and microsurgical repair. The drugs were administered intraperitoneally at the dose of 50 mg/kg/d for 28 and 56 d postoperatively. At the end of treatment, the motor function recovery of the peroneal component of the sciatic nerve was assessed and the regenerated nerves were analysed morphometrically on histological semi-thin sections. Also, the reinnervated extensor digitorum longus (EDL) muscles were studied histochemically using the adenosine-triphosphatase (ATP-ase) technique 56 d after surgery. Motor function assessment at 56 d after nerve repair revealed that L-acetylcarnitine-treated animals recovered a clinical grade significantly higher (p less than 0.05) than the control animals. Twenty-eight days after nerve repair, the number of myelinated fibres was significantly higher (p less than 0.05) in L-acetylcarnitine and ganglioside-treated animals than in control animals. However, 56 d after nerve repair the number of regenerated fibres in all the drug-treated groups was not significantly different from that of the control group. The EDL muscles of the drug-treated animals did not show significant differences from those of control animals with respect to fibre composition and fibre diameter although the L-acetylcarnitine-treated animals exhibited a significantly lower (p less than 0.05) degree of muscle atrophy than did the control animals. The results of the present work seem to indicate that L-acetylcarnitine and to a lesser extent gangliosides exert some favourable effect on the regeneration of the transected sciatic nerve in rats.(ABSTRACT TRUNCATED AT 250 WORDS)
对三种药物制剂，即左旋肉碱、左旋乙酰肉碱和神经节苷脂，进行了测试，以考察它们增强大鼠坐骨神经切断并显微外科修复后再生的能力。术后28天和56天，以50毫克/千克/天的剂量腹腔注射这些药物。治疗结束时，评估坐骨神经腓总神经成分的运动功能恢复情况，并在组织学半薄切片上对再生神经进行形态计量分析。此外，术后56天，使用三磷酸腺苷酶（ATP酶）技术对重新神经支配的趾长伸肌（EDL）进行组织化学研究。神经修复后56天的运动功能评估显示，接受左旋乙酰肉碱治疗的动物恢复的临床分级明显高于对照组动物（p小于0.05）。神经修复28天后，接受左旋乙酰肉碱和神经节苷脂治疗的动物中，有髓纤维数量明显多于对照组动物（p小于0.05）。然而，神经修复56天后，所有药物治疗组的再生纤维数量与对照组没有显著差异。尽管接受左旋乙酰肉碱治疗的动物肌肉萎缩程度明显低于对照组动物（p小于0.05），但药物治疗动物的EDL肌肉在纤维组成和纤维直径方面与对照动物没有显著差异。本研究结果似乎表明，左旋乙酰肉碱以及程度稍轻的神经节苷脂对大鼠切断的坐骨神经再生有一些有利作用。（摘要截选至250字）
