College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science, Yangtze University, Jingzhou 434025, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Carbohydr Polym. 2015 May 5;121:477-85. doi: 10.1016/j.carbpol.2014.12.016. Epub 2014 Dec 30.
Nanogels (NGs) with drug tracking and delivery possess promising usage in clinical treatment. In this study, an available, low toxic and facile approach was developed to synthesize CdTe quantum dots loaded nanogels (QDs-NGs). The QDs-NGs retained the intrinsic pH sensitivity of the QDs with regard to the fluorescence intensity. The QDs-NGs were easily internalized by the cells as fluorescence probes, and acted as carriers for delivering methotrexate (MTX). The cellular uptake indicated that the QDs-NGs can protect QDs from decomposition in cytoplasm and retain the native fluorescence intensity. MTT assay demonstrated that the QDs-NGs greatly decreased the cytotoxicity of the QDs. The MTX loaded QDs-NGs exhibited slow release property in PBS buffer. Moreover, the MTX loaded QDs-NGs distinctly enhanced the availability of drug. The QDs-NGs are potential nanocarriers for the cell imaging and drug delivery.
载药纳米凝胶具有药物跟踪和输送的功能，在临床治疗中有很好的应用前景。在本研究中，开发了一种可行的、低毒的、简单的方法来合成载量子点纳米凝胶（QDs-NGs）。与量子点的荧光强度相比，QDs-NGs 保留了内在的 pH 敏感性。作为荧光探针，QDs-NGs 很容易被细胞内化，并作为甲氨蝶呤（MTX）的载体。细胞摄取表明，QDs-NGs 可以防止 QDs 在细胞质中分解，并保持其固有荧光强度。MTT 试验表明，QDs-NGs 大大降低了 QDs 的细胞毒性。载 QDs-NGs 在 PBS 缓冲液中表现出缓慢释放特性。此外，载 QDs-NGs 的 MTX 明显提高了药物的有效性。QDs-NGs 是用于细胞成像和药物输送的潜在纳米载体。
