Jenkins Rowena, Wootton Mandy, Howe Robin, Cooper Rose
Department of Biomedical Sciences, Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff, CF5 2YB, Wales, UK,
Arch Microbiol. 2015 May;197(4):597-601. doi: 10.1007/s00203-015-1091-6. Epub 2015 Feb 14.
Pseudomonas and Burkholderia pose a significant health threat to people with chronic respiratory conditions; the resistance inherent in these bacteria indicates that new antimicrobial strategies are required. Susceptibility of 56 strains of P. aeruginosa and 55 strains of Burkholderia to manuka honey, tobramycin and colistin using microbroth dilution and E strip was determined. MICs of antibiotics with honey were determined to search for synergistic combinations against two representative strains of each genus. All strains exhibited susceptibility to honey ≤10 % (w/v); mean susceptibility of Burkholderia (4.6 % w/v) was lower than P. aeruginosa (7.3 % w/v). Synergistic or additive combinations were found with all four strains tested. Combinations of manuka honey with antibiotics can be used to lower the MIC need to successfully inhibit both P. aeruginosa and B. cepacia. The use of honey as a combination agent may be possible for the management of P. aeruginosa and B. cepacia.
假单胞菌和伯克霍尔德菌对患有慢性呼吸道疾病的人构成重大健康威胁；这些细菌固有的耐药性表明需要新的抗菌策略。使用微量肉汤稀释法和E试验条测定了56株铜绿假单胞菌和55株伯克霍尔德菌对麦卢卡蜂蜜、妥布霉素和黏菌素的敏感性。测定了抗生素与蜂蜜组合的最低抑菌浓度（MIC），以寻找针对每个属的两种代表性菌株的协同组合。所有菌株对蜂蜜的敏感性均≤10%（w/v）；伯克霍尔德菌的平均敏感性（4.6%w/v）低于铜绿假单胞菌（7.3%w/v）。在所测试的所有四种菌株中均发现了协同或相加组合。麦卢卡蜂蜜与抗生素的组合可用于降低成功抑制铜绿假单胞菌和洋葱伯克霍尔德菌所需的MIC。将蜂蜜用作联合用药可能对铜绿假单胞菌和洋葱伯克霍尔德菌的治疗有效。
