Biomaterials Department, National Research Centre, Dokki, Cairo, Egypt.
Biomaterials Department, National Research Centre, Dokki, Cairo, Egypt ; Physics Department, Faculty of Science, El-Taif University, Saudi Arabia.
J Adv Res. 2014 Mar;5(2):201-8. doi: 10.1016/j.jare.2013.02.004. Epub 2013 Mar 28.
The present work aims to study the in vitro properties of nano-hydroxyapatite/chitosan-gelatin composite materials. In vitro behavior was performed in simulated body fluid (SBF) to verify the formation of apatite layer onto the composite surfaces. The in vitro data proved the deposition of calcium and phosphorus ions onto hydroxyapatite /polymeric composite surfaces especially those containing high concentrations of polymer content. The degradation of the composites decreased with increase in the polymeric matrix content and highly decreased in the presence of citric acid (CA), especially these composites which contain 30% polymeric content. The water absorption of the composites increased with increase in the polymeric content and highly increased with CA addition. The Fourier transformed infrared reflectance (FT-IR) and scanning electron microscope (SEM) for the composites confirmed the formation of bone-like apatite layer on the composite surfaces, especially those containing high content of polymers (30%) with 0.2 M of CA. These promising composites have suitable properties for bio-applications such as bone grafting and bone tissue engineering applications in the future.
本工作旨在研究纳米羟基磷灰石/壳聚糖-明胶复合材料的体外性能。在模拟体液（SBF）中进行体外行为研究，以验证在复合表面上形成磷灰石层。体外数据证明了钙和磷离子沉积在羟基磷灰石/聚合物复合材料表面上，特别是那些含有高浓度聚合物含量的复合材料。随着聚合物基质含量的增加，复合材料的降解减少，而在柠檬酸（CA）存在下，复合材料的降解大大减少，特别是那些含有 30%聚合物含量的复合材料。复合材料的吸水率随聚合物含量的增加而增加，而随着 CA 加入量的增加而大大增加。复合材料的傅里叶变换红外反射（FT-IR）和扫描电子显微镜（SEM）证实了在复合表面上形成了类骨磷灰石层，特别是那些含有高含量聚合物（30%）和 0.2 M CA 的复合材料。这些有前途的复合材料具有适合生物应用的特性，例如骨移植和骨组织工程应用。
