Seldin M F, Howard T A, D'Eustachio P
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Genomics. 1989 Jul;5(1):24-8. doi: 10.1016/0888-7543(89)90082-7.
Progeny from an interspecific backcross between laboratory mice and Mus spretus were typed for inheritance of eight genetic markers on chromosome 12. Marker order determined by segregation analyses of 115 meiotic events was in good agreement with that determined previously using intraspecific laboratory strain backcrosses. Two additional markers, D12Nyu5 and Lamb-1, previously not ordered, were located in the middle of the interval between D12Nyu12 and D12Nyu1. Marker spacing was reduced in the interspecific cross relative to that observed in intraspecific crosses. Furthermore, the interspecific cross was characterized by marked deviation from 1:1 segregation in the recombinant chromosomes and very strong positive interference. These data suggest that comparisons of different mouse crosses may facilitate the understanding of underlying mechanisms that govern recombination events in complex genomes.
对实验室小鼠与西班牙小鼠种间回交产生的后代进行了12号染色体上8个遗传标记的遗传分型。通过对115个减数分裂事件的分离分析确定的标记顺序，与之前使用种内实验室品系回交确定的顺序高度一致。另外两个之前未排序的标记D12Nyu5和Lamb-1，位于D12Nyu12和D12Nyu1之间区间的中部。与种内杂交相比，种间杂交中标记间距减小。此外，种间杂交的特征是重组染色体中明显偏离1:1分离，且存在非常强的正干扰。这些数据表明，比较不同的小鼠杂交可能有助于理解复杂基因组中控制重组事件的潜在机制。
