Matsuda Y, Manly K F, Chapman V M
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263.
Mamm Genome. 1993 Sep;4(9):475-80. doi: 10.1007/BF00364780.
The analysis of major satellite sequence differences between Mus spretus and laboratory mice provides a robust method for analyzing the centromere location for the genetic maps of each mouse chromosome. Fluorescence in situ hybridization (FISH) of a genomic probe, pMR196, for the laboratory mouse major satellite sequences was used to identify C57BL/6Ros (B6) pericentromeric heterochromatin in progeny of reciprocal backcross matings. These included 80 (B6 x M. spretus)F1 x M. spretus progeny (BSS) and 70 (B6 x M. spretus)F1 x B6 (BSB) progeny. FISH analysis of pericentromeric heterochromatin was conducted on the same metaphase spreads that were karyotypically analyzed for chromosome-specific banding patterns. Analysis of chromosomal segregation suggested that there was not primary deviation from random assortment during meiosis in the interspecific hybrid female, because nearly all of the 190 pair-wise comparisons did not deviate from expected and because there was no consistent pattern of deviation of the same chromosomes in the reciprocal backcross progeny from similar (C57BL/6 x M. spretus)F1 hybrid females. These results affirm the value of using the major satellite to genetically mark pericentromeric heterochromatin in the analysis of the segregation and assortment of centromeres in Mus interspecific crosses.
对家鼠和实验小鼠之间主要卫星序列差异的分析，为分析每条小鼠染色体遗传图谱的着丝粒位置提供了一种可靠的方法。使用针对实验小鼠主要卫星序列的基因组探针pMR196进行荧光原位杂交（FISH），以鉴定正反交回交后代中C57BL/6Ros（B6）着丝粒周围的异染色质。这些后代包括80只（B6×家鼠）F1×家鼠后代（BSS）和70只（B6×家鼠）F1×B6（BSB）后代。对着丝粒周围异染色质的FISH分析是在与进行染色体特异性带型核型分析相同的中期染色体铺片上进行的。染色体分离分析表明，种间杂交雌性减数分裂过程中没有明显偏离随机分配，因为几乎所有190对比较都未偏离预期，且正反交回交后代中相同染色体的偏离模式与相似的（C57BL/6×家鼠）F1杂交雌性不一致。这些结果证实了在小家鼠种间杂交中分析着丝粒的分离和分配时，利用主要卫星对着丝粒周围异染色质进行遗传标记的价值。
