枸杞多糖可保护人视网膜色素上皮细胞免受氧化应激诱导的细胞凋亡。
Lycium barbarum polysaccharides protected human retinal pigment epithelial cells against oxidative stress-induced apoptosis.
作者信息
Liu Lian, Lao Wei, Ji Qing-Shan, Yang Zhi-Hao, Yu Guo-Cheng, Zhong Jing-Xiang
机构信息
Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
出版信息
Int J Ophthalmol. 2015 Feb 18;8(1):11-6. doi: 10.3980/j.issn.2222-3959.2015.01.02. eCollection 2015.
AIM
To investigate the protective effect and its mechanism of lycium barbarum polysaccharides (LBP) against oxidative stress-induced apoptosis in human retinal pigment epithelial cells.
METHODS
ARPE-19 cells, a human retinal pigment epithelial cell lines, were exposed to different concentrations of H2O2 for 24h, then cell viability was measured by Cell Counting Kit-8 (CCK-8) assay to get the properly concentration of H2O2 which can induce half apoptosis of APRE-19. With different concentrations of LBP pretreatment, the ARPE-19 cells were then exposed to appropriate concentration of H2O2, cell apoptosis was detected by flow cytometric analysis. Expression levels of Bcl-2 and Bax were measured by real time quantitative polymerase chain reaction (RT-PCR) technique.
RSULTS
LBP significantly reduced the H2O2-induced ARPE-19 cells' apoptosis. LBP inhibited the H2O2-induced down-regulation of Bcl-2 and up-regulation of Bax.
CONCLUSION
LBP could protect ARPE-19 cells from H2O2-induced apoptosis. The Bcl-2 family had relationship with the protective effects of LBP.
目的
探讨枸杞多糖（LBP）对氧化应激诱导的人视网膜色素上皮细胞凋亡的保护作用及其机制。
方法
将人视网膜色素上皮细胞系ARPE-19细胞暴露于不同浓度的H2O2中24小时，然后通过细胞计数试剂盒-8（CCK-8）检测细胞活力，以获得能诱导ARPE-19细胞半数凋亡的合适H2O2浓度。用不同浓度的LBP预处理后，再将ARPE-19细胞暴露于合适浓度的H2O2中，通过流式细胞术分析检测细胞凋亡情况。采用实时定量聚合酶链反应（RT-PCR）技术检测Bcl-2和Bax的表达水平。
结果
LBP显著降低了H2O2诱导的ARPE-19细胞凋亡。LBP抑制了H2O2诱导的Bcl-2下调和Bax上调。
结论
LBP可保护ARPE-19细胞免受H2O2诱导的凋亡。Bcl-2家族与LBP的保护作用有关。
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