Li Qinghe, Wang Yuanyuan, Hu Xiaoxiang, Zhao Yaofeng, Li Ning
1] Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China [2] State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100193, China [3] National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing, China.
1] Department of Biological Sciences, Bengbu Medical College, Bengbu 233030, China [2] State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100193, China [3] National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing, China.
Sci Rep. 2015 Mar 4;5:8748. doi: 10.1038/srep08748.
It is well-known that environment influences DNA methylation, however, the extent of heritable DNA methylation variation following animal domestication remains largely unknown. Using meDIP-chip we mapped the promoter methylomes for 23,316 genes in muscle tissues of ancestral and domestic chickens. We systematically examined the variation of promoter DNA methylation in terms of different breeds, differentially expressed genes, SNPs and genes undergo genetic selection sweeps. While considerable changes in DNA sequence and gene expression programs were prevalent, we found that the inter-strain DNA methylation patterns were highly conserved in promoter region between the wild and domestic chicken breeds. Our data suggests a global preservation of DNA methylation between the wild and domestic chicken breeds in either a genome-wide or locus-specific scale in chick muscle tissues.
众所周知，环境会影响DNA甲基化，然而，动物驯化后可遗传的DNA甲基化变异程度仍 largely未知。我们使用甲基化DNA免疫沉淀芯片（meDIP-chip）绘制了原鸡和家鸡肌肉组织中23316个基因的启动子甲基化组图谱。我们系统地研究了不同品种、差异表达基因、单核苷酸多态性（SNPs）以及经历遗传选择清除的基因在启动子DNA甲基化方面的变异情况。虽然DNA序列和基因表达程序普遍存在相当大的变化，但我们发现野生和家鸡品种之间的品系间DNA甲基化模式在启动子区域高度保守。我们的数据表明，在雏鸡肌肉组织中，野生和家鸡品种之间在全基因组或基因座特异性水平上都存在DNA甲基化的整体保留。
