Yamamura Keisuke, Miura Tadashi, Kou I, Muramatsu Takashi, Furusawa Masahiro, Yoshinari Masao
Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502; Department of Endodontics and Clinical Cariology, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku 101-0061, Japan.
Dent Mater J. 2015;34(1):120-7. doi: 10.4012/dmj.2014-076. Epub 2015 Jan 17.
The purpose of this study was to investigate the influence of superhydrophilic treatments of titanium on the behavior of osteoblastlike cells. Superhydrophilic specimens were prepared with sandblast and acid-etching (DW), oxygen plasma (Plasma) and ultraviolet light (UV), and were stored in distilled water for 3 days immediately after these treatments. Specimens stored in air for 3 weeks were used as a control Air group. Initial cell attachment, proliferation, alkaline phosphatase activity, and osteocalcin secretion of mouse osteoblast-like cells MC3T3-E1 were enhanced more on superhydrophilic groups than were Air specimens. On confocal laser scanning microscope images of cell morphology, the expression of actin filaments was observed on the superhydrophilic groups, whereas relatively little actin filament expression was seen on the Air surfaces on all culture periods. These results indicate that DW, Plasma, or UV treatment has potential for the creation and maintenance of superhydrophilic surfaces and the enhancement of the initial attachment, proliferation, and differentiation of osteoblast-like cells.
本研究的目的是探讨钛的超亲水处理对成骨样细胞行为的影响。采用喷砂和酸蚀（DW）、氧等离子体（Plasma）和紫外线（UV）制备超亲水标本，并在处理后立即在蒸馏水中保存3天。在空气中保存3周的标本用作对照空气组。与空气标本相比，超亲水组小鼠成骨样细胞MC3T3-E1的初始细胞附着、增殖、碱性磷酸酶活性和骨钙素分泌增强更为明显。在共聚焦激光扫描显微镜下观察细胞形态图像，在所有培养期内，超亲水组均观察到肌动蛋白丝的表达，而在空气表面肌动蛋白丝表达相对较少。这些结果表明，DW、Plasma或UV处理具有创造和维持超亲水表面以及增强成骨样细胞初始附着、增殖和分化的潜力。
