Yang Su-De, Wang Wei, Li Jia-Chun, Xiao Wei
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(20):3955-7.
HPLC fingerprint of Congrong Zonggan capsule was established in order to provide basis for quality evaluation. With acteoside as the reference, HPLC was adopted for fingerprint analysis on Congrong Zonggan capsule. The chromatographic conditions wereas follows. Waters C18 column (4.6 mm x 150 mm, 5 μm) was used, with methylalcohol-0.1% formic acid as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 330 nm, and the column temperature was 30 °C. This method was highly accurate and reproducible. All of the 13 components in tested samples reached the baseline resolved peak, and 15 batches of finished products showed the similarity of above 0.95. The method was accurate and feasible and could be used as a simple and effective method to evaluate the quality of the traditional Chinese medicines.
为了给质量评价提供依据，建立了苁蓉总苷胶囊的高效液相色谱指纹图谱。以毛蕊花糖苷为对照品，采用高效液相色谱法对苁蓉总苷胶囊进行指纹图谱分析。色谱条件如下：采用Waters C18柱（4.6 mm×150 mm，5 μm），以甲醇-0.1%甲酸为流动相进行梯度洗脱，流速为1.0 mL·min⁻¹。检测波长为330 nm，柱温为30℃。该方法准确性高、重现性好。供试品中13个成分的峰均达到基线分离，15批成品相似度均在0.95以上。该方法准确可行，可作为评价该中药质量的简便有效方法。
