Stärk G, Stauff J, Miltenburger H G, Stumm-Fischer I
Mutat Res. 1985 Jan-Feb;155(1-2):27-33. doi: 10.1016/0165-1218(85)90021-7.
The effect of irradiation with wavelengths of 320-418 nm on direct-acting mutagenicity of 1-nitropyrene (1-NP) and particulate-matter extracts of a direct-injecting diesel engine was examined. The activity of samples in the Ames test with and without addition of S9 mix in Salmonella typhimurium strain TA98, TA100 and TA1538 decreased with increasing irradiation energy. Visible light was sufficient to destroy the mutagenicity of a 0.1-mM 1-NP solution. The same was true for particulate matter crude extracts as well as the transitional and oxygenate subfractions isolated by column chromatography. UV spectroscopy, thin-layer chromatography and GC-MS analysis were performed to characterize the irradiation products of 1-NP. The mechanism of photodecomposition of 1-NP at different wavelengths and the significance of this finding for the evaluation of health risks from diesel vehicles are discussed.
研究了波长为320 - 418 nm的辐射对1-硝基芘（1-NP）的直接致突变性以及直喷式柴油发动机颗粒物提取物的影响。在鼠伤寒沙门氏菌TA98、TA100和TA1538菌株中，添加和不添加S9混合物的Ames试验中，样品的活性随着辐射能量的增加而降低。可见光足以破坏0.1 mM 1-NP溶液的致突变性。颗粒物粗提物以及通过柱色谱分离的过渡和含氧亚组分也是如此。进行了紫外光谱法、薄层色谱法和气相色谱 - 质谱分析以表征1-NP的辐照产物。讨论了1-NP在不同波长下的光解机制以及这一发现对评估柴油车辆健康风险的意义。
