易于使用的基于塑料光纤的丁醛检测生物传感器。
Easy to use plastic optical fiber-based biosensor for detection of butanal.
作者信息
Cennamo Nunzio, Di Giovanni Stefano, Varriale Antonio, Staiano Maria, Di Pietrantonio Fabio, Notargiacomo Andrea, Zeni Luigi, D'Auria Sabato
机构信息
Department of Industrial and Information Engineering, SUN, Aversa, Italy.
Institute of Protein Biochemistry, CNR, Napoli, Italy.
出版信息
PLoS One. 2015 Mar 19;10(3):e0116770. doi: 10.1371/journal.pone.0116770. eCollection 2015.
The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.
这项工作的最终目标是通过实现一种简单、小型且低成本的实验方法来实现对丁醛的选择性检测。为此，将一种猪源气味结合蛋白与塑料光纤工具中的表面等离子体共振传感技术相结合，通过竞争分析实现对丁醛的选择性检测。这有助于降低传感装置的成本和尺寸，并为设计“芯片实验室”平台提供了可能性。所得结果表明，该系统方法能够在20μM至1000μM的浓度范围内选择性地检测出丁醛的存在。
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