Earl Patricia L, Americo Jeffrey L, Moss Bernard
Laboratory of Viral Diseases, 33 North Drive, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, Unites States.
Laboratory of Viral Diseases, 33 North Drive, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, Unites States.
Virology. 2015 Jul;481:161-5. doi: 10.1016/j.virol.2015.02.048. Epub 2015 Mar 17.
Previously, we screened 38 inbred mouse strains for susceptibility to monkeypox virus (MPXV) and focused on wild-derived CAST mice because of their extreme vulnerability. Here, we provide further analysis of inbred mouse strains. NZW/Lac and C58 mice exhibited more weight loss than other classical inbred strains but all survived intranasal challenges with 10(4) to 10(6)PFU of MPXV. Mice from three wild derived strains, in addition to CAST, exhibited severe weight loss and died or were euthanized. LD50 values for CASA, MOLF and PERA were 100, 6800 and >10(5)PFU, respectively. CASA was inbred independently from the same founders as CAST, whereas MOLF and PERA are genetically and geographically distinct. The MPXV susceptibility of the F1 progeny of CAST and either C57BL/6 or BALB/c indicated that resistance is dominant. Back-crossing the F1 progeny of C57BL/6 and CAST to CAST suggested more than one independent resistant locus.
此前，我们对38种近交系小鼠品系进行了猴痘病毒（MPXV）易感性筛选，并重点关注野生来源的CAST小鼠，因为它们极易感染。在此，我们对近交系小鼠品系进行了进一步分析。NZW/Lac和C58小鼠比其他经典近交系品系表现出更多体重减轻，但经10⁴至10⁶PFU的MPXV鼻内攻击后均存活。除CAST外，来自三个野生来源品系的小鼠表现出严重体重减轻并死亡或被安乐死。CASA、MOLF和PERA的半数致死剂量（LD50）值分别为100、6800和>10⁵PFU。CASA与CAST由相同的奠基者独立培育而来，而MOLF和PERA在遗传和地理上均有差异。CAST与C57BL/6或BALB/c的F1代子代对MPXV的易感性表明抗性是显性的。将C57BL/6和CAST的F1代子代回交至CAST表明存在不止一个独立的抗性位点。
