Ebrahimi Azizollah, Hemati Majid, Shabanpour Ziba, Habibian Dehkordi Saeed, Bahadoran Shahab, Lotfalian Sharareh, Khubani Shahin
Department of Pathobiology, School of Veterinary Sciences, Shahrekord University, Shahrekord, IR Iran.
School of Veterinary Sciences, Shahrekord University, Shahrekord, IR Iran.
Jundishapur J Microbiol. 2015 Feb 20;8(2):e16058. doi: 10.5812/jjm.16058. eCollection 2015 Feb.
Resistance toward quaternary ammonium compounds (QACs) is widespread among a diverse range of microorganisms and is facilitated by several mechanisms such as biofilm formation.
In this study, the effects of benzalkonium chloride on planktonic growth and biofilm formation by some field isolates of animal bacterial pathogens were investigated.
Forty clinical isolates of Escherichia coli, Salmonella serotypes, Staphylococcus aureus and Streptococcus agalactiae (10 isolates of each) were examined for effects of benzalkonium chloride on biofilm formation and planktonic growth using microtiter plates. For all the examined strains in the presence of benzalkonium chloride, biofilm development and planktonic growth were affected at the same concentrations of disinfectant.
The means of strains growth increase after the minimal inhibitory concentration (MIC) were significant in all the bacteria (except for E. coli in 1/32 and S. agalactiae in of 1/8 MIC). Biofilm formation increased with decrease of antiseptics concentration; a significant increase was found in all the samples. The most turbidity related to S. aureus and the least to Salmonella.
Bacterial resistance against quaternary ammonium compounds is increasing which can increase the bacterial biofilm formation.
对季铵化合物（QACs）的耐药性在多种微生物中广泛存在，并且通过生物膜形成等多种机制得以促进。
在本研究中，调查了苯扎氯铵对一些动物细菌病原体的现场分离株的浮游生长和生物膜形成的影响。
使用微量滴定板检测40株临床分离的大肠杆菌、沙门氏菌血清型、金黄色葡萄球菌和无乳链球菌（每种10株），以研究苯扎氯铵对生物膜形成和浮游生长的影响。对于所有检测菌株，在苯扎氯铵存在的情况下，相同浓度的消毒剂会影响生物膜形成和浮游生长。
在所有细菌中（除1/32 MIC浓度下的大肠杆菌和1/8 MIC浓度下的无乳链球菌外），最低抑菌浓度（MIC）后菌株生长增加的平均值具有显著性。生物膜形成随着防腐剂浓度的降低而增加；在所有样本中均发现显著增加。与金黄色葡萄球菌相关的浊度最高，与沙门氏菌相关的浊度最低。
细菌对季铵化合物的耐药性正在增加，这可能会增加细菌生物膜的形成。
