Maloney Jenny, Keselman Aleksander, Li Erqiu, Singer Steven M
Georgetown University, Dept. of Biology, 37th and O Sts NW Reiss 406, Washington DC 20057, USA.
Microbes Infect. 2015 Jun;17(6):462-7. doi: 10.1016/j.micinf.2015.03.006. Epub 2015 Mar 19.
Nitric oxide (NO) has been shown to inhibit Giardia lamblia in vitro and in vivo. This study sought to determine if Giardia infection induces arginase 1 (ARG1) expression in host macrophages to reduce NO production. Stimulations of RAW 264.7 macrophage-like cells with Giardia extract induced arginase activity. Real-time PCR and immunohistochemistry showed increased ARG1 and nitric oxide synthase 2 (NOS2) expression in mouse intestine following infection. Flow cytometry demonstrated increased numbers of macrophages positive for both ARG1 and NOS2 in lamina propria following infection, but there was no evidence of increased expression of ARG1 in these cells.
一氧化氮（NO）已被证明在体外和体内均可抑制蓝氏贾第鞭毛虫。本研究旨在确定贾第虫感染是否会诱导宿主巨噬细胞中精氨酸酶1（ARG1）的表达，以减少NO的产生。用贾第虫提取物刺激RAW 264.7巨噬细胞样细胞可诱导精氨酸酶活性。实时PCR和免疫组织化学显示，感染后小鼠肠道中ARG1和一氧化氮合酶2（NOS2）的表达增加。流式细胞术表明感染后固有层中ARG1和NOS2均呈阳性的巨噬细胞数量增加，但没有证据表明这些细胞中ARG1的表达增加。
