George V Cijo, Kumar D R Naveen, Suresh P K, Kumar R Ashok
School of Bio Sciences and Technology, VIT University, Vellore, India.
Department of Zoology, Government Arts College, Dharmapuri, 636705 Tamil Nadu India.
J Food Sci Technol. 2015 Apr;52(4):2328-35. doi: 10.1007/s13197-014-1289-7. Epub 2014 Feb 25.
Annona muricata is a naturally occurring edible plant with wide array of therapeutic potentials. In India, it has a long history of traditional use in treating various ailments. The present investigation was carried out to characterize the phytochemicals present in the methanolic and aqueous leaf extracts of A. muricata, followed by validation of its radical scavenging and DNA protection activities. The extracts were also analyzed for its total phenolic contents and subjected to HPLC analysis to determine its active metabolites. The radical scavenging activities were premeditated by various complementary assays (DRSA, FRAP and HRSA). Further, its DNA protection efficacy against H2O2 induced toxicity was evaluated using pBR322 plasmid DNA. The results revealed that the extracts were highly rich in various phytochemicals including luteolin, homoorientin, tangeretin, quercetin, daidzein, epicatechin gallate, emodin and coumaric acid. Both the extracts showed significant (p < 0.05) radical scavenging activities, while methanolic extract demonstrated improved protection against H2O2-induced DNA damage when compared to aqueous extract. A strong positive correlation was observed for the estimated total phenolic contents and radical scavenging potentials of the extracts. Further HPLC analysis of the phyto-constituents of the extracts provides a sound scientific basis for compound isolation.
刺果番荔枝是一种天然可食用植物，具有广泛的治疗潜力。在印度，它在治疗各种疾病方面有着悠久的传统应用历史。本研究旨在对刺果番荔枝叶的甲醇提取物和水提取物中的植物化学成分进行表征，随后验证其自由基清除和DNA保护活性。还对提取物的总酚含量进行了分析，并进行了HPLC分析以确定其活性代谢物。通过各种互补测定法（DRSA、FRAP和HRSA）来测定自由基清除活性。此外，使用pBR322质粒DNA评估其对H2O2诱导毒性的DNA保护功效。结果表明，提取物富含多种植物化学成分，包括木犀草素、高车前苷、橘皮素、槲皮素、大豆苷元、表儿茶素没食子酸酯、大黄素和香豆酸。两种提取物均表现出显著（p < 0.05）的自由基清除活性，而与水提取物相比，甲醇提取物对H2O2诱导的DNA损伤表现出更好的保护作用。提取物的估计总酚含量与自由基清除潜力之间观察到强烈的正相关。提取物植物成分的进一步HPLC分析为化合物分离提供了可靠的科学依据。
