Mestres Gemma, Espanol Montserrat, Xia Wei, Persson Cecilia, Ginebra Maria-Pau, Ott Marjam Karlsson
Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.
Biomaterials, Biomechanics and Tissue Engineering, Dpt. Materials Science and Metallurgy, Technical University of Catalonia, Barcelona, Spain.
PLoS One. 2015 Apr 2;10(3):e0120381. doi: 10.1371/journal.pone.0120381. eCollection 2015.
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.
白细胞与生物材料接触后的增殖和活化在炎症反应程度中起关键作用，而炎症反应程度随后可能决定植入生物材料的临床失败或成功。本研究的目的是评估纳米和微结构仿生羟基磷灰石基质是否会影响巨噬细胞样细胞的生长和活化。对由片状和针状晶体的缠结网络组成的具有不同晶体形态的羟基磷灰石基质进行了评估。在材料表面（直接接触）以及提取物中，即由材料改性的培养基（间接接触）中评估巨噬细胞增殖。此外, 还研究了在提取物中补充钙离子和/或蛋白质的效果。通过量化活性氧的释放和形态学观察来评估基质上的巨噬细胞活化。结果表明，基质微观结构的差异在巨噬细胞活化中起主要作用，因为与针状基质上几乎不存在活性氧释放相比，在片状晶体基质上培养巨噬细胞后活性氧释放更高。然而，巨噬细胞增殖的差异归因于基质中不同的离子交换和蛋白质吸附/保留，而不是材料的质地。
