Byun Hayeon, Jang Gyu Nam, Hong Min-Ho, Yeo Jiwon, Shin Hyunjung, Kim Won Jong, Shin Heungsoo
Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, Republic of Korea.
BK21 FOUR Education and Research Group for Biopharmaceutical Innovation Leader, Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, Republic of Korea.
Nano Converg. 2022 Oct 10;9(1):47. doi: 10.1186/s40580-022-00338-2.
Bone healing involves complex processes including inflammation, induction, and remodeling. In this context, anti-inflammatory and osteoconductive multi-functional nanoparticles have attracted considerable attention for application in improved bone tissue regeneration. In particular, nanoparticles that promote suppression of inflammatory response after injury and direction of desirable tissue regeneration events are of immense interest to researchers. We herein report a one-step method to prepare multi-functional nanoparticles using tannic acid (TA) and simulated body fluid (SBF) containing multiple mineral ions. Mineral-tannic acid nanoparticles (mTNs) were rapidly fabricated in 10 min, and their size (around 250-350 nm) and chemical composition were controlled through the TA concentration. In vitro analysis using human adipose derived stem cells (hADSCs) showed that mTNs effectively scavenged reactive oxygen species (ROS) and enhanced osteogenesis of hADSCs by inducing secretion of alkaline phosphatase. mTNs also increased osteogenic marker gene expression even in the presence of ROS, which can generally arrest osteogenesis (OPN: 1.74, RUNX2: 1.90, OCN: 1.47-fold changes relative to cells not treated with mTNs). In vivo analysis using a mouse peritonitis model revealed that mTNs showed anti-inflammatory effects by decreasing levels of pro-inflammatory cytokines in blood (IL-6: 73 ± 4, TNF-α: 42 ± 2%) and peritoneal fluid (IL-6: 78 ± 2, TNF-α: 21 ± 6%). We believe that this one-step method for fabrication of multi-functional nanoparticles has considerable potential in tissue engineering approaches that require control of complex microenvironments, as required for tissue regeneration.
骨愈合涉及包括炎症、诱导和重塑在内的复杂过程。在此背景下，具有抗炎和骨传导功能的多功能纳米颗粒在改善骨组织再生中的应用引起了广泛关注。特别是，能够促进损伤后炎症反应抑制和引导理想组织再生事件的纳米颗粒，受到了研究人员的极大关注。我们在此报告一种使用单宁酸（TA）和含有多种矿物质离子的模拟体液（SBF）制备多功能纳米颗粒的一步法。在10分钟内快速制备了矿物-单宁酸纳米颗粒（mTNs），并通过TA浓度控制其尺寸（约250-350nm）和化学成分。使用人脂肪来源干细胞（hADSCs）进行的体外分析表明，mTNs有效地清除了活性氧（ROS），并通过诱导碱性磷酸酶的分泌增强了hADSCs的成骨作用。即使在存在通常会抑制成骨作用的ROS的情况下，mTNs也能增加成骨标记基因的表达（OPN：1.74，RUNX2：1.90，OCN：相对于未用mTNs处理的细胞有1.47倍变化）。使用小鼠腹膜炎模型进行的体内分析表明，mTNs通过降低血液（IL-6：73±4，TNF-α：42±2%）和腹腔液（IL-6：78±2，TNF-α：21±6%）中促炎细胞因子的水平而显示出抗炎作用。我们认为，这种制备多功能纳米颗粒的一步法在需要控制复杂微环境的组织工程方法中具有巨大潜力，这是组织再生所必需的。
