Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
Applied Enzyme Technology Ltd., Gwent Group Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UK.
N Biotechnol. 2015 Sep 25;32(5):511-20. doi: 10.1016/j.nbt.2015.03.011. Epub 2015 Apr 6.
This paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes. End-point and real-time PCR, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of magnetic particles in these approaches. Although the immunomagnetic separation for capturing single bacteria from contaminating microflora and interfering food components has demonstrated to improve the performance on these approaches, the integration of magnetic particles for multiplex detection of bacteria is still in a preliminary stage and requires further studies.
本文探讨了用于多重检测食源性病原体的新方法，特别关注三种与食品爆发相关的最常见病原体：沙门氏菌、大肠杆菌 O157:H7 和李斯特菌。终点和实时 PCR、经典免疫技术、生物传感器、微阵列和微流控平台以及用于多重检测食源性病原体的商业试剂盒将得到回顾，特别关注磁性颗粒在这些方法中的作用。虽然免疫磁分离可从污染的微生物群和干扰食物成分中捕获单个细菌，从而提高这些方法的性能，但磁性颗粒用于多重细菌检测的集成仍处于初步阶段，需要进一步研究。
