Sheikhzadeh Farzam, Etemad Asieh, Khoshghadam Sahar, Asl Naser Ahmadi, Zare Peyman
Department of Animal Physiology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran,
Neurol Sci. 2015 Jul;36(7):1163-6. doi: 10.1007/s10072-015-2208-z. Epub 2015 Apr 10.
The hippocampus is a region in the brain that is crucial for learning and memory. Previous researches proved that brain-derived neurotrophic factor (BDNF) is a probable responsible protein in the learning and memory formation process. BDNF content is thought to be affected by environmental enrichment and physical activity. The purpose of this research was to identify the effect of short- and long-term forced exercise on hippocampal BDNF levels. A total of 30 Wistar rats were randomly divided into three groups (control, short-term exercise and long-term exercise) and treated by treadmill running based on their group. As the treadmill running period finished, the animals were anesthetized. The hippocampus was dissected out immediately and BDNF content of the samples was assessed by ELISA. None of the exercise paradigms did make any significant change on hippocampal BDNF levels. Although exercise was proposed to up-regulate BDNF levels, these results show that the intensity or the duration of running paradigm used in forced exercise protocols here was not enough to affect BDNF levels in the hippocampus significantly.
海马体是大脑中对学习和记忆至关重要的一个区域。先前的研究证明，脑源性神经营养因子（BDNF）可能是学习和记忆形成过程中的一种关键蛋白。BDNF的含量被认为会受到环境丰富度和体育活动的影响。本研究的目的是确定短期和长期强迫运动对海马体中BDNF水平的影响。总共30只Wistar大鼠被随机分为三组（对照组、短期运动组和长期运动组），并根据分组进行跑步机跑步处理。当跑步机跑步期结束后，将动物麻醉。立即取出海马体，通过酶联免疫吸附测定法（ELISA）评估样本中的BDNF含量。没有任何一种运动模式对海马体BDNF水平产生显著变化。尽管有人提出运动可上调BDNF水平，但这些结果表明，此处强迫运动方案中使用的跑步模式的强度或持续时间不足以显著影响海马体中的BDNF水平。
