Corrêa Jôice Dias, Pereira Daniele Sirineu, Madeira Mila Fernandes Moreira, Queiroz-Junior Celso Martins, Souza Danielle Glória, Teixeira Mauro Martins, Costa José Eustáquio, Teixeira Antônio Lúcio, da Silva Tarcília Aparecida
Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Antonio Carlos Avenue 6627, 31.270-901 Belo Horizonte, MG, Brazil.
Department of Physical Therapy, School of Physical Education, Physical Therapy and Occupational Therapy, Federal University of Minas Gerais, Antonio Carlos Avenue 6627, 31.270-901 Belo Horizonte, MG, Brazil.
Mediators Inflamm. 2014;2014:373765. doi: 10.1155/2014/373765. Epub 2014 Dec 21.
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophic factor family. Outside the nervous system, BDNF has been shown to be expressed in various nonneural tissues, such as periodontal ligament, dental pulp, and odontoblasts. Although a role for BDNF in periodontal regeneration has been suggested, a function for BDNF in periodontal disease has not yet been studied. The aim of this study was to analyze the BDNF levels in periodontal tissues of patients with chronic periodontitis (CP) and periodontally healthy controls (HC). All subjects were genotyped for the rs4923463 and rs6265 BDNF polymorphisms. Periodontal tissues were collected for ELISA, myeloperoxidase (MPO), and microscopic analysis from 28 CP patients and 29 HC subjects. BDNF levels were increased in CP patients compared to HC subjects. A negative correlation was observed when analyzing concentration of BDNF and IL-10 in inflamed periodontium. No differences in frequencies of BDNF genotypes between CP and HC subjects were observed. However, BDNF genotype GG was associated with increased levels of BDNF, TNF-α, and CXCL10 in CP patients. In conclusion, BDNF seems to be associated with periodontal disease process, but the specific role of BDNF still needs to be clarified.
脑源性神经营养因子（BDNF）是神经营养因子家族的一员。在神经系统之外，BDNF已被证明在各种非神经组织中表达，如牙周韧带、牙髓和成牙本质细胞。尽管已有研究表明BDNF在牙周再生中发挥作用，但BDNF在牙周疾病中的功能尚未得到研究。本研究的目的是分析慢性牙周炎（CP）患者和牙周健康对照者（HC）牙周组织中的BDNF水平。对所有受试者进行rs4923463和rs6265 BDNF基因多态性分型。收集28例CP患者和29例HC受试者的牙周组织进行酶联免疫吸附测定（ELISA）、髓过氧化物酶（MPO）检测和显微镜分析。与HC受试者相比，CP患者的BDNF水平升高。分析炎症牙周组织中BDNF和白细胞介素-10（IL-10）的浓度时，观察到两者呈负相关。未观察到CP患者和HC受试者之间BDNF基因型频率的差异。然而，BDNF基因型GG与CP患者中BDNF、肿瘤坏死因子-α（TNF-α）和CXC趋化因子配体10（CXCL10）水平的升高有关。总之，BDNF似乎与牙周疾病进程相关，但BDNF的具体作用仍有待阐明。
