Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark;
Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
J Extracell Vesicles. 2015 Apr 8;4:26048. doi: 10.3402/jev.v4.26048. eCollection 2015.
Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.
细胞外囊泡 (EVs) 和外泌体很难从生物体液中富集或纯化，因此对这些囊泡进行定量和表型分析既繁琐又不准确。Jørgensen 等人提出的 EV 微阵列 (J Extracell Vesicles, 2013; 2: 10) 是一种多重、高灵敏度和高通量的平台，对 EV 表面标志物的分析和分子分析能力进行了改进。在此，我们提出了一种扩展的微阵列平台，用于检测和表型分析多达 60 种抗原的血浆衍生 EV（针对外泌体进行了优化），而无需在分析前进行任何富集或纯化。
