Blumberg Mark S, Sokoloff Greta, Tiriac Alexandre, Del Rio-Bermudez Carlos
Department of Psychology, The University of Iowa, Iowa City, IA, 52242; Department of Biology, The University of Iowa, Iowa City, IA, 52242.
Dev Psychobiol. 2015 May;57(4):506-17. doi: 10.1002/dev.21305. Epub 2015 Apr 11.
Neurophysiological recording of brain activity has been critically important to the field of neuroscience, but has contributed little to the field of developmental psychobiology. The reasons for this can be traced largely to methodological difficulties associated with recording neural activity in behaving newborn rats and mice. Over the last decade, however, the evolution of methods for recording from head-fixed newborns has heralded a new era in developmental neurophysiology. Here, we review these recent developments and provide a step-by-step primer for those interested in applying the head-fix method to their own research questions. Until now, this method has been used primarily to investigate spontaneous brain activity across sleep and wakefulness, the contributions of the sensory periphery to brain activity, or intrinsic network activity. Now, with some ingenuity, the uses of the head-fix method can be expanded to other domains to benefit our understanding of brain-behavior relations under normal and pathophysiological conditions across early development.
大脑活动的神经生理学记录对神经科学领域至关重要，但对发育心理生物学领域贡献甚微。造成这种情况的原因很大程度上可追溯到与记录新生大鼠和小鼠行为时的神经活动相关的方法学困难。然而，在过去十年中，用于固定头部的新生动物记录方法的发展开创了发育神经生理学的新纪元。在此，我们回顾这些最新进展，并为有兴趣将头部固定方法应用于自身研究问题的人员提供一份详细指南。到目前为止，该方法主要用于研究睡眠和清醒状态下的自发脑活动、感觉外周对脑活动的贡献或内在网络活动。现在，只要有一定的创新思维，头部固定方法的应用范围就可以扩展到其他领域，以有助于我们理解早期发育过程中正常和病理生理条件下的脑-行为关系。
