MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Biomaterials. 2015;53:522-31. doi: 10.1016/j.biomaterials.2015.02.126. Epub 2015 Mar 21.
Recently, cycling hypoxia, with cycles of hypoxia followed by reoxygenation, was found to induce the up-regulation of HIF-1 activity in tumor cells and reduce the success rate of radiotherapy or chemotherapy. Thus, the ability to visualize cycling hypoxia in cells and in vivo is of great significance. To address this critical need, we have developed ruthenium(II) anthraquinone complexes as reversible two-photon luminescent probes; these probes are well suited for selectively and dynamically monitoring cycling hypoxia in live cells. Moreover, the probes can conveniently visualize inner hypoxia in 3D multicellular tumor spheroids and detect repeated cycles of hypoxia-reoxygenation in living zebrafish via two-photon luminescent imaging. The present study provides a powerful luminescent imaging tool for dynamically sensing of cycling hypoxia in vivo.
最近，发现周期性缺氧（缺氧循环后再复氧）会诱导肿瘤细胞中 HIF-1 活性上调，降低放疗或化疗的成功率。因此，能够在细胞内和体内可视化周期性缺氧具有重要意义。为了满足这一关键需求，我们开发了钌（II）蒽醌配合物作为可逆的双光子发光探针；这些探针非常适合选择性和动态监测活细胞中的周期性缺氧。此外，通过双光子荧光成像，探针可以方便地可视化 3D 多细胞肿瘤球体中的内部缺氧，并检测活体斑马鱼中重复的缺氧-复氧循环。本研究为动态感应体内周期性缺氧提供了一种强大的发光成像工具。
