Huang Yan-Qiang, Huang Gan-Rong, Wu Ming-Hui, Tang Hua-Ying, Huang Zan-Song, Zhou Xi-Han, Yu Wen-Qiang, Su Jian-Wei, Mo Xiao-Qiang, Chen Bing-Pu, Zhao Li-Juan, Huang Xiao-Feng, Wei Hong-Yu, Wei Lian-Deng
Yan-Qiang Huang, Gan-Rong Huang, Hua-Ying Tang, Xiao-Qiang Mo, Bing-Pu Chen, Li-Juan Zhao, Xiao-Feng Huang, Hong-Yu Wei, Lian-Deng Wei, Department of Medical Microbiology and Immunology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China.
World J Gastroenterol. 2015 Apr 14;21(14):4225-31. doi: 10.3748/wjg.v21.i14.4225.
To investigate the inhibitory effects of emodin, baicalin, etc. on the hefA gene of multidrug resistance (MDR) in Helicobacter pylori (H. pylori).
The double dilution method was used to screen MDR H. pylori strains and determine the minimum inhibitory concentrations (MICs) of emodin, baicalin, schizandrin, berberine, clarithromycin, metronidazole, tetracycline, amoxicillin and levofloxacin against H. pylori strains. After the screened MDR stains were treated with emodin, baicalin, schizandrin or berberine at a 1/2 MIC concentration for 48 h, changes in MICs of amoxicillin, tetracycline, levofloxacin, metronidazole and clarithromycin were determined. MDR strains with reduced MICs of amoxicillin were selected to detect the hefA mRNA expression by real-time quantitative PCR.
A total of four MDR H. pylori strains were screened. Treatment with emodin, baicalin, schizandrin and berberine significantly decreased the MICs of amoxicillin and tetracycline against some strains, decreased by 1 to 2 times, but did not significantly change the MICs of clarithromycin, levofloxacin, and metronidazole against MDR strains. In the majority of strains with reduced MICs of amoxicillin, hefA mRNA expression was decreased; one-way ANOVA (SPSS 12.0) used for comparative analysis, P < 0.05.
Emodin, baicalin, schizandrin and berberine significantly decreased the MICs of amoxicillin and tetracycline against some H. pylori strains, possibly by mechanisms associated with decreasing hefA mRNA expression.
研究大黄素、黄芩苷等对幽门螺杆菌（H. pylori）多药耐药（MDR）的hefA基因的抑制作用。
采用双倍稀释法筛选MDR幽门螺杆菌菌株，并测定大黄素、黄芩苷、五味子素、黄连素、克拉霉素、甲硝唑、四环素、阿莫西林和左氧氟沙星对幽门螺杆菌菌株的最低抑菌浓度（MICs）。将筛选出的MDR菌株用1/2 MIC浓度的大黄素、黄芩苷、五味子素或黄连素处理48小时后，测定阿莫西林、四环素、左氧氟沙星、甲硝唑和克拉霉素的MICs变化。选择阿莫西林MICs降低的MDR菌株，通过实时定量PCR检测hefA mRNA表达。
共筛选出4株MDR幽门螺杆菌菌株。大黄素、黄芩苷、五味子素和黄连素处理可使部分菌株的阿莫西林和四环素MICs显著降低，降低1至2倍，但对克拉霉素、左氧氟沙星和甲硝唑对MDR菌株的MICs无显著影响。在大多数阿莫西林MICs降低的菌株中，hefA mRNA表达降低；采用单因素方差分析（SPSS 12.0）进行比较分析，P < 0.05。
大黄素、黄芩苷、五味子素和黄连素可使部分幽门螺杆菌菌株的阿莫西林和四环素MICs显著降低，可能与降低hefA mRNA表达的机制有关。
