Zhang R, Zhou M, Tu Y, Zhang N F, Deng K D, Ma T, Diao Q Y
Feed Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing, China.
College of Animal Science, Jinling Institute of Technology, Nanjing, Jiangsu, China.
J Anim Physiol Anim Nutr (Berl). 2016 Feb;100(1):33-8. doi: 10.1111/jpn.12338. Epub 2015 Apr 28.
This study aimed to investigate the effect of dietary supplementation with Lactobacillus plantarum and Bacillus subtilis on growth performance, apparent nutrient digestibility and stress-related indicators in dairy calves. Twenty-four neonatal Holstein calves were randomly allocated to three treatments: a basal diet with no supplementation (control), the basal diet supplemented with 1.7 × 10(10) CFU per head per day (CFU/h.d) of L. plantarum GF103 (LB group) or the basal diet supplemented with a mixture of L. plantarum GF103 (1.7 × 10(10) CFU/h.d) and B. subtilis B27 (1.7 × 10(8) CFU/h.d) (LBS group). Dry matter intake (DMI), average daily gain (ADG), feed conversation ratio (FCR), apparent digestibility of nutrients and stress-related indicators were measured in this trail. The result indicated that no significant differences were observed in DMI or ADG (p > 0.05), but the FCR was improved in the LB group over the first 12 weeks (p > 0.05). The apparent digestibility of nutrients was not altered by probiotics in week 6 (p > 0.05), but the apparent digestibility of total phosphorus was significantly greater in the LB and LBS groups in week 8 (p > 0.05); additionally, an increase in the apparent digestibility of crude protein was detected in the LBS group (p > 0.05). Oral administration of L. plantarum alone improved the T-lymphocyte transformation rate on days 58 and 62 (p > 0.05), while adding the mixture of L. plantarum and B. subtilis increased the T-lymphocyte transformation rate (p > 0.05) but decreased the content of cortisol on day 58 (p > 0.05). No significant differences were detected between the LB and LBS groups in growth performance, apparent digestibility of nutrients and stress-related indicators (p > 0.05). The results suggested that oral administration of L. plantarum improved growth performance, nutrient digestibility and relieved weaning stress in calves, but no additional effect was obtained by supplementation with B. subtilis.
本研究旨在探讨日粮中添加植物乳杆菌和枯草芽孢杆菌对犊牛生长性能、表观养分消化率及应激相关指标的影响。将24头新生荷斯坦犊牛随机分为三组：不添加任何物质的基础日粮组（对照组）、基础日粮中添加1.7×10(10) CFU/头·天（CFU/h.d）植物乳杆菌GF103的组（LB组）或基础日粮中添加植物乳杆菌GF103（1.7×10(10) CFU/h.d）与枯草芽孢杆菌B27（1.7×10(8) CFU/h.d）混合物的组（LBS组）。在本试验中测定了干物质采食量（DMI）、平均日增重（ADG）、饲料转化率（FCR）、养分表观消化率及应激相关指标。结果表明，DMI或ADG未观察到显著差异（p>0.05），但LB组在第12周内FCR得到改善（p>0.05）。益生菌在第6周时未改变养分表观消化率（p>0.05），但在第8周时，LB组和LBS组总磷表观消化率显著更高（p>0.05）；此外，LBS组粗蛋白表观消化率有所提高（p>0.05）。单独口服植物乳杆菌可提高第58天和第62天的T淋巴细胞转化率（p>0.05），而添加植物乳杆菌和枯草芽孢杆菌的混合物可提高T淋巴细胞转化率（p>0.05），但在第58天时降低了皮质醇含量（p>0.05）。LB组和LBS组在生长性能、养分表观消化率及应激相关指标方面未检测到显著差异（p>0.05）。结果表明，口服植物乳杆菌可改善犊牛的生长性能、养分消化率并缓解断奶应激，但添加枯草芽孢杆菌未获得额外效果。
