El-Gendy Reem, Kirkham Jennifer, Newby Phillipa J, Mohanram Yamuna, Boccaccini Aldo Roberto, Yang Xuebin B
1 Biomaterials and Tissue Engineering Group, Department of Oral Biology, University of Leeds, St. James's University Hospital , Leeds, United Kingdom .
2 Biomineralisation Group, Department of Oral Biology, University of Leeds, St. James's University Hospital , Leeds, United Kingdom .
Tissue Eng Part A. 2015 Jul;21(13-14):2034-43. doi: 10.1089/ten.tea.2014.0485. Epub 2015 Apr 29.
Identification of a suitable cell source combined with an appropriate 3D scaffold is an essential prerequisite for successful engineering of skeletal tissues. Both osteogenesis and angiogenesis are key processes for bone regeneration. This study investigated the vascularization potential of a novel combination of human dental pulp stromal cells (HDPSCs) with 45S5 Bioglass® scaffolds for tissue-engineered mineral constructs in vivo and in vitro. 45S5 Bioglass scaffolds were produced by the foam replication technique with the standard composition of 45 wt% SiO2, 24.5 wt% Na2O, 24.5 wt% CaO, and 6 wt% P2O5. HDPSCs were cultured in monolayers and on porous 45S5 Bioglass scaffolds under angiogenic and osteogenic conditions for 2-4 weeks. HDPSCs expressed endothelial gene markers (CD34, CD31/PECAM1, and VEGFR2) under both conditions in the monolayer. A combination of HDPSCs with 45S5 Bioglass enhanced the expression of these gene markers. Positive immunostaining for CD31/PECAM1 and VEGFR2 and negative staining for CD34 supported the gene expression data, while histology revealed evidence of endothelial cell-like morphology within the constructs. More organized tubular structures, resembling microvessels, were seen in the constructs after 8 weeks of implantation in vivo. In conclusion, this study suggests that the combination of HDPSCs with 45S5 Bioglass scaffolds offers a promising strategy for regenerating vascularized bone grafts.
识别合适的细胞来源并结合适当的三维支架是成功构建骨骼组织工程的必要前提。成骨和血管生成都是骨再生的关键过程。本研究调查了人牙髓基质细胞（HDPSCs）与45S5生物活性玻璃®支架的新型组合在体内和体外对组织工程化矿物质构建体的血管化潜力。45S5生物活性玻璃支架采用泡沫复制技术制备，其标准组成为45 wt% SiO2、24.5 wt% Na2O、24.5 wt% CaO和6 wt% P2O5。HDPSCs在单层培养以及在血管生成和成骨条件下于多孔45S5生物活性玻璃支架上培养2至4周。HDPSCs在单层培养的两种条件下均表达内皮基因标志物（CD34、CD31/PECAM1和VEGFR2）。HDPSCs与45S5生物活性玻璃的组合增强了这些基因标志物的表达。CD31/PECAM1和VEGFR2的阳性免疫染色以及CD34的阴性染色支持了基因表达数据，而组织学显示构建体内存在内皮细胞样形态的证据。在体内植入8周后，构建体中可见更有序的类似微血管的管状结构。总之，本研究表明HDPSCs与45S5生物活性玻璃支架的组合为再生血管化骨移植提供了一种有前景的策略。
