Detsch Rainer, Alles Sonja, Hum Jasmin, Westenberger Peter, Sieker Frank, Heusinger Dominik, Kasper Cornelia, Boccaccini Aldo R
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
J Biomed Mater Res A. 2015 Mar;103(3):1029-37. doi: 10.1002/jbm.a.35238. Epub 2014 Jun 14.
In the context of bone tissue engineering (BTE), combinations of bioactive scaffolds with living cells are investigated to optimally yield functional bone tissue for implantation purposes. Bioactive glasses are a class of highly bioactive, inorganic materials with broad application potential in BTE strategies. The aim of this study was to evaluate bioactive glass (45S5 Bioglass(®)) samples of composition: 45 SiO2, 24.5 CaO, 24.5 Na2O, and 6 P2O5 (in wt%) as scaffold materials for mesenchymal stem cells (MSC). Pore architecture of the scaffolds as well as cell behavior in the three-dimensional environment was evaluated by several methods. Investigations concerned the osteogenic cell attachment, growth and differentiation of adipose tissue derived MSC (adMSC) compared with MSC from human full term umbilical cord tissues (ucMSC) on porous Bioglass(®)-based scaffolds over a cultivation period of 5 weeks. Differences in lineage-specific osteogenic differentiation of adMSC and ucMSC on Bioglass(®) samples were demonstrated. The investigation led to positive results in terms of cell attachment, proliferation, and differentiation of MSC onto Bioglass(®)-based scaffolds confirming the relevance of these matrices for BTE applications.
在骨组织工程（BTE）领域，人们研究生物活性支架与活细胞的组合，以优化生成用于植入目的的功能性骨组织。生物活性玻璃是一类具有高生物活性的无机材料，在BTE策略中具有广泛的应用潜力。本研究的目的是评估成分（按重量百分比计）为45 SiO₂、24.5 CaO、24.5 Na₂O和6 P₂O₅的生物活性玻璃（45S5 Bioglass®）样品作为间充质干细胞（MSC）的支架材料。通过多种方法评估了支架的孔隙结构以及细胞在三维环境中的行为。研究了脂肪组织来源的MSC（adMSC）与人足月脐带组织来源的MSC（ucMSC）在多孔Bioglass®基支架上培养5周期间的成骨细胞附着、生长和分化情况。结果表明，adMSC和ucMSC在Bioglass®样品上的谱系特异性成骨分化存在差异。该研究在MSC在Bioglass®基支架上的细胞附着、增殖和分化方面取得了积极成果，证实了这些基质在BTE应用中的相关性。
