Choi Jee-Hyun, Lee Jin-Ho, Oh Byung-Keun, Choi Jeong-Woo
J Nanosci Nanotechnol. 2014 Aug;14(8):5658-61. doi: 10.1166/jnn.2014.8830.
Localized surface plasmon resonance (LSPR) is the phenomenon that is observed on specific metal nanoparticles (NPs) like Au, Ag which can be used for sensitive detection for many kinds of biomaterials. Dopamine (DA) is a typical neurotransmitter considered as indicator of some neural diseases. Due to its small size, it is very difficult to detect DA at low concentrations directly and sensitively with conventional sensing techniques. In this research, we propose a DA detection sensor based on LSPR phenomenon. Electrochemical deposition technique was used to make LSPR substrates, where Au NPs were electrochemically deposited on ITO glasses and these substrates showed optical characteristic of LSPR phenomenon. Different concentrations of DA solution were deposited on antibody immobilized LSPR substrates. With additions of increasing concentrations of DA, LSPR peak intensity was increased linearly. These results could be applied to many fields of clinical trials for diseases caused by small molecules.
局域表面等离子体共振（LSPR）是在诸如金、银等特定金属纳米颗粒（NPs）上观察到的现象，可用于多种生物材料的灵敏检测。多巴胺（DA）是一种典型的神经递质，被视为某些神经疾病的指标。由于其尺寸小，使用传统传感技术直接且灵敏地检测低浓度的多巴胺非常困难。在本研究中，我们提出了一种基于LSPR现象的多巴胺检测传感器。采用电化学沉积技术制作LSPR基底，其中金纳米颗粒被电化学沉积在ITO玻璃上，这些基底表现出LSPR现象的光学特性。将不同浓度的多巴胺溶液沉积在固定有抗体的LSPR基底上。随着多巴胺浓度的增加，LSPR峰强度呈线性增加。这些结果可应用于由小分子引起的疾病的许多临床试验领域。
