Jose Mini, Tollis Sylvain, Nair Deepak, Mitteau Romain, Velours Christophe, Massoni-Laporte Aurelie, Royou Anne, Sibarita Jean-Baptiste, McCusker Derek
Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France Centre for Neuroscience, Indian Institute of Science, Bangalore 560012, India.
Dynamics of Cell Growth and Division, European Institute of Chemistry and Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
Mol Biol Cell. 2015 Jul 1;26(13):2519-34. doi: 10.1091/mbc.E14-11-1527. Epub 2015 May 6.
The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key network hub, rich in genetic interactions with endocytic and exocytic components. Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and interspersed endocytic and exocytic domains compared with control cells. These data are consistent with an important role for the exocyst in coordinating endocytosis and exocytosis.
内吞作用与胞吐作用的偶联是从受精到神经元活动和细胞极性等基本生物学过程的基础。然而，控制内吞作用和胞吐作用空间组织的机制仍需阐明。我们利用出芽酵母中基于定量成像的筛选方法，鉴定出89个在其中一条或两条途径定位上存在缺陷的突变体。高分辨率单囊泡追踪显示，内吞和胞吐突变体she4∆和bud6∆以相反的方式改变了高尔基体后囊泡的动力学。在内吞作用和胞吐作用途径在极性建立过程中表现出强烈的相互依赖性，而在极性维持过程中则更为独立。系统分析确定外排体复合物是一个关键的网络枢纽，与内吞和胞吐成分有丰富的遗传相互作用。与对照细胞相比，外排体突变体表现出内吞和高尔基体后囊泡动力学的改变以及内吞和胞吐结构域的交错分布。这些数据与外排体在协调内吞作用和胞吐作用中的重要作用相一致。
