Resch Yvonne, Michel Sven, Kabesch Michael, Lupinek Christian, Valenta Rudolf, Vrtala Susanne
Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Department of Pediatric Pneumology and Allergy, University Children's Hospital Regensburg (KUNO), Regensburg, Germany.
J Allergy Clin Immunol. 2015 Oct;136(4):1083-91. doi: 10.1016/j.jaci.2015.03.024. Epub 2015 May 5.
House dust mites (HDMs) represent one of the most important inducers of respiratory allergies worldwide.
We sought to investigate the IgE and IgG reactivity profiles to a comprehensive panel of HDM allergens in children with allergic asthma and to compare them with those of nonasthmatic atopic children.
Sera from clinically well-characterized asthmatic children with HDM allergy (n = 105), nonasthmatic children (n = 53), and nonatopic nonasthmatic children (n = 53) were analyzed for IgE and IgG reactivity to a panel of 7 HDM allergens (nDer p 1, rDer p 2, rDer p 5, rDer p 7, rDer p 10, rDer p 21, and rDer p 23) by means of allergen microarray technology.
Asthmatic children with HDM allergy more frequently showed an IgE response to each of the HDM allergens and recognized more allergens than nonasthmatic children with HDM allergy. Furthermore, IgE levels to certain HDM allergens (nDer p 1, P = .002; rDer p 2, P = .007; rDer p 5, P = .031; and rDer p 23, P < .001) were significantly higher in asthmatic children than in children without asthma. By contrast, fewer asthmatic children showed IgG reactivity to HDM allergens than nonasthmatic children, but allergen-specific IgG levels were comparable.
The IgE and IgG reactivity profiles to HDM allergens, as well as IgE levels to certain allergen components, differed considerably between children with and without asthmatic symptoms caused by HDM allergy. In fact, asthmatic children were characterized by an expanded IgE repertoire regarding the numbers of recognized allergen components and by increased specific IgE levels.
屋尘螨（HDM）是全球范围内呼吸道过敏最重要的诱发因素之一。
我们试图研究过敏性哮喘儿童对一组全面的屋尘螨变应原的IgE和IgG反应谱，并将其与非哮喘性特应性儿童的反应谱进行比较。
采用变应原微阵列技术，分析临床特征明确的对屋尘螨过敏的哮喘儿童（n = 105）、非哮喘儿童（n = 53）和非特应性非哮喘儿童（n = 53）血清中对7种屋尘螨变应原（nDer p 1、rDer p 2、rDer p 5、rDer p 7、rDer p 10、rDer p 21和rDer p 23）的IgE和IgG反应。
对屋尘螨过敏的哮喘儿童比同样对屋尘螨过敏的非哮喘儿童更频繁地对每种屋尘螨变应原表现出IgE反应，且识别的变应原更多。此外，哮喘儿童对某些屋尘螨变应原（nDer p 1，P = .002；rDer p 2，P = .007；rDer p 5，P = .031；rDer p 23，P < .001）的IgE水平显著高于非哮喘儿童。相比之下，对屋尘螨变应原表现出IgG反应的哮喘儿童比非哮喘儿童少，但变应原特异性IgG水平相当。
有或无由屋尘螨过敏引起的哮喘症状的儿童之间，对屋尘螨变应原的IgE和IgG反应谱以及对某些变应原成分的IgE水平存在相当大的差异。事实上，哮喘儿童的特征是识别的变应原成分数量增加导致IgE反应谱扩大以及特异性IgE水平升高。
