Brglez Josipa, Nikolov Pavel, Angelin Alessandro, Niemeyer Christof M
Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
Chemistry. 2015 Jun 22;21(26):9440-6. doi: 10.1002/chem.201500086. Epub 2015 May 14.
The modification of the backbone properties of DNA origami nanostructures through noncovalent interactions with designed intercalators, based on acridine derivatized with side chains containing esterified fatty acids or oligo(ethylene glycol) residues is reported. Spectroscopic analyses indicate that these intercalators bind to DNA origami structures. Atomic force microscopy studies reveal that intercalator binding does not affect the structural intactness but leads to altered surface properties of the highly negatively charged nanostructures, as demonstrated by their interaction with solid mica or graphite supports. Moreover, the noncovalent interaction between the intercalators and the origami structures leads to alteration in cellular uptake, as shown by confocal microscopy studies using two different eukaryotic cell lines. Hence, the intercalator approach offers a potential means for tailoring the surface properties of DNA nanostructures.
报道了通过与基于含有酯化脂肪酸或聚乙二醇残基侧链的吖啶衍生的设计嵌入剂进行非共价相互作用来修饰DNA折纸纳米结构的主链性质。光谱分析表明这些嵌入剂与DNA折纸结构结合。原子力显微镜研究表明，嵌入剂的结合不会影响结构完整性，但会导致高负电荷纳米结构的表面性质发生改变，这通过它们与固体云母或石墨载体的相互作用得到证明。此外，如使用两种不同真核细胞系的共聚焦显微镜研究所表明的，嵌入剂与折纸结构之间的非共价相互作用导致细胞摄取的改变。因此，嵌入剂方法为定制DNA纳米结构的表面性质提供了一种潜在手段。
