Cao Hung, Kang Bong Jin, Lee Chia-An, Shung K Kirk, Hsiai Tzung K
IEEE Rev Biomed Eng. 2015;8:114-24. doi: 10.1109/RBME.2015.2431681. Epub 2015 May 11.
Inadequate replacement of lost ventricular myocardium from myocardial infarction leads to heart failure. Investigating the regenerative capacity of mammalian hearts represents an emerging direction for tissue engineering and cell-based therapy. Recent advances in stem cells hold promise to restore cardiac functions. However, embryonic or induced pluripotent stem cell-derived cardiomyocytes lack functional phenotypes of the native myocardium, and transplanted tissues are not fully integrated for synchronized electrical and mechanical coupling with the host. In this context, this review highlights the mechanical and electrical strategies to promote cardiomyocyte maturation and integration, and to assess the functional phenotypes of regenerating myocardium. Simultaneous microelectrocardiogram and high-frequency ultrasound techniques will also be introduced to assess electrical and mechanical coupling for small animal models of heart regeneration.
心肌梗死导致的心室心肌丢失未能得到充分替代会引发心力衰竭。研究哺乳动物心脏的再生能力是组织工程和基于细胞的治疗的一个新兴方向。干细胞领域的最新进展有望恢复心脏功能。然而，胚胎干细胞或诱导多能干细胞衍生的心肌细胞缺乏天然心肌的功能表型，移植组织也不能完全整合以与宿主进行同步的电和机械耦合。在此背景下，本综述重点介绍促进心肌细胞成熟和整合以及评估再生心肌功能表型的机械和电策略。还将介绍同步心电图和高频超声技术，以评估心脏再生小动物模型的电和机械耦合。
