Gil-Sanz Cristina, Espinosa Ana, Fregoso Santiago P, Bluske Krista K, Cunningham Christopher L, Martinez-Garay Isabel, Zeng Hongkui, Franco Santos J, Müller Ulrich
Molecular and Cellular Neuroscience Department, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
Graduate Program in Cell Biology, Stem Cells and Development, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Neuron. 2015 May 20;86(4):1091-1099. doi: 10.1016/j.neuron.2015.04.019.
Using genetic fate-mapping with Cux2-Cre and Cux2-CreERT2 mice we demonstrated that the neocortical ventricular zone (VZ) contains radial glial cells (RGCs) with restricted fate potentials (Franco et al., 2012). Using the same mouse lines, Guo et al. (2013) concluded that the neocortical VZ does not contain lineage-restricted RGCs. We now show that the recombination pattern in Cux2-Cre/CreERT2 mice depends on genetic background and breeding strategies. We provide evidence that Guo et al. likely reached different conclusions because they worked with transgenic sublines with drifted transgene expression patterns. In Cux2-Cre and Cux2-CreERT2 mice that recapitulate the endogenous Cux2 expression pattern, the vast majority of fate-mapped neurons express Satb2 but not Ctip2, confirming that a restricted subset of all neocortical projection neurons belongs to the Cux2 lineage. This Matters Arising paper is in response to Guo et al. (2013), published in Neuron. See also the Matters Arising Response paper by Eckler et al. (2015), published concurrently with this Matters Arising in Neuron.
利用Cux2-Cre和Cux2-CreERT2小鼠进行遗传命运图谱分析，我们证明了新皮质脑室区（VZ）含有具有有限命运潜能的放射状胶质细胞（RGCs）（Franco等人，2012年）。使用相同的小鼠品系，Guo等人（2013年）得出结论，新皮质VZ不包含谱系受限的RGCs。我们现在表明，Cux2-Cre/CreERT2小鼠中的重组模式取决于遗传背景和育种策略。我们提供的证据表明，Guo等人可能得出了不同的结论，因为他们使用的转基因亚系的转基因表达模式发生了漂移。在重现内源性Cux2表达模式的Cux2-Cre和Cux2-CreERT2小鼠中，绝大多数命运映射的神经元表达Satb2但不表达Ctip2，证实所有新皮质投射神经元的一个受限子集属于Cux2谱系。这篇“问题提出”论文是对发表在《神经元》杂志上的Guo等人（2013年）的回应。另见Eckler等人（2015年）同时发表在《神经元》杂志上的“问题提出回应”论文。
