Biology Department, Faculty of Mathematics & Natural Sciences, Brawijaya University, Indonesia; Biology Department, Faculty of Mathematics & Natural Sciences, University of Jember, Indonesia.
Biology Department, Faculty of Mathematics & Natural Sciences, University of Jember, Indonesia.
J Infect Public Health. 2015 Nov-Dec;8(6):575-82. doi: 10.1016/j.jiph.2015.04.022. Epub 2015 Jun 6.
Humans develop anti-salivary proteins after arthropod bites or exposure to insect salivary proteins. This reaction indicates that vector bites have a positive effect on the host immune response, which can be used as epidemiological markers of exposure to the vector. Our previous study identified two immunogenic proteins with molecular weights of 31 kDa and 56 kDa from salivary gland extract (SGE) of Aedes aegypti that cross-reacted with serum samples from Dengue Hemorrhagic Fever (DHF) patients and healthy people in an endemic area (Indonesia). Serum samples from individuals living in non-endemic area (sub-tropical country) and infants did not show the immunogenic reactions. The objective of this research was to identify two immunogenic proteins, i.e., 31 and 56 kDa by using proteomic analysis. In this study, proteomic analysis resulted in identification of 13 proteins and 7 proteins from the 31 kDa- and 56 kDa-immunogenic protein bands, respectively. Among those proteins, the D7 protein (Arthropode Odorant-Binding Protein, AOBP) was the most abundant in 31-kDa band, and apyrase was the major protein of the 56-kDa band.
人类在被节肢动物叮咬或接触昆虫唾液蛋白后会产生抗唾液蛋白。这种反应表明，媒介叮咬对宿主的免疫反应有积极影响，可作为接触媒介的流行病学标志物。我们之前的研究从埃及伊蚊唾液腺提取物 (SGE) 中鉴定出两种具有 31 kDa 和 56 kDa 分子量的免疫原性蛋白，它们与登革热出血热 (DHF) 患者和流行地区（印度尼西亚）健康人群的血清样本发生交叉反应。来自非流行地区（亚热带国家）和婴儿的个体的血清样本未显示出免疫反应。本研究的目的是通过蛋白质组学分析鉴定两种免疫原性蛋白，即 31 kDa 和 56 kDa。在这项研究中，蛋白质组学分析分别鉴定出 31 kDa 和 56 kDa 免疫蛋白带中的 13 种和 7 种蛋白。在这些蛋白质中，D7 蛋白（节肢动物气味结合蛋白，AOBP）是 31 kDa 带中最丰富的，而磷酸酶是 56 kDa 带中的主要蛋白。
