Mazzei M, Nardini R, Verin R, Forzan M, Poli A, Tolari F
Department of Veterinary Science, University of Pisa, Pisa, Italy.
New Microbes New Infect. 2015 Jun 4;7:41-7. doi: 10.1016/j.nmni.2015.05.008. eCollection 2015 Sep.
The aim of this study was to further investigate the role of wild boar (Sus scrofa) as a reservoir for hepatitis E virus (HEV). Sixty-four blood and faecal samples collected from wild boar hunted in Central Italy in 2011-2012 were examined by indirect enzyme-linked immunosorbent assay and RT-PCR analysis. Positive RT-PCR samples were further examined by nucleotide sequence determination and subsequent phylogenetic analysis. Thirty-six sera (56.2%) were positive for HEV-specific antibodies, and six (9.4%) faecal samples scored RT-PCR-positive results. Four animals were positive by both enzyme-linked immunosorbent assay and RT-PCR. Phylogenetic analysis showed that the detected wild boar-derived HEV sequences clustered within genotype 3, with similarity to sequences of human origin collected in a nearby area in 2012. Our data confirm that HEV is endemic in the wild boar population in the research area and that these wild animals could play an important role in the epidemiology of HEV infection.
本研究的目的是进一步调查野猪（Sus scrofa）作为戊型肝炎病毒（HEV）宿主的作用。通过间接酶联免疫吸附试验和逆转录聚合酶链反应（RT-PCR）分析，对2011年至2012年在意大利中部捕获的64份野猪血液和粪便样本进行了检测。对RT-PCR阳性样本进一步进行核苷酸序列测定和后续的系统发育分析。36份血清（56.2%）戊型肝炎病毒特异性抗体呈阳性，6份（9.4%）粪便样本RT-PCR检测结果为阳性。4只动物酶联免疫吸附试验和RT-PCR均呈阳性。系统发育分析表明，检测到的野猪源戊型肝炎病毒序列聚集在3型基因型内，与2012年在附近地区采集的人类源序列相似。我们的数据证实，戊型肝炎病毒在研究区域的野猪种群中呈地方流行，并且这些野生动物可能在戊型肝炎病毒感染的流行病学中发挥重要作用。
