Lee Ji Hee, Kang Joo Won, Shin Soon Bum, Seong Chi Nam
Aquaculture Industry Research Division, South Sea Fisheries Research Institute, National Institute of Fisheries Science South Sea Fisheries Research Institute (NIFS), Yeosu 59780, Republic of Korea.
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3540-3546. doi: 10.1099/ijsem.0.002161. Epub 2017 Sep 4.
An aerobic, gliding and yellow-pigmented bacterium, designated strain SFD31T, was isolated from brown alga collected from the South Sea, Republic of Korea. Cells were Gram-stain-negative, and catalase- and oxidase-positive. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain SFD31T forms an independent lineage within the genus Winogradskyella. Strain SFD31T was related distantly to Winogradskyella echinorum KMM 6211T (97.9 %, 16S rRNA gene sequence similarity), Winogradskyellalitoriviva KMM 6491T (97.4 %), Winogradskyellapulchriflava EM106T (97.2 %) and Winogradskyellaeckloniae EC29T (96.9 %). The major fatty acids of strain SFD31T were iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and unknown 13.565. The only isoprenoid quinone of the isolate was menaquinone 6. The major polar lipids were phosphatidylethanolamine, four unidentified aminolipids and two unidentified lipids. The DNA G+C content of strain SFD31T was 36.0 mol%. Phenotypic characteristics distinguished strain SFD31T from the related species of the genus Winogradskyella. On the basis of the evidence presented in this study, a novel species, Winogradskyella flava sp. nov., is proposed for strain SFD31T (=KCTC 52348T=JCM 31798T).
从韩国南海采集的褐藻中分离出一株需氧、滑行且产黄色色素的细菌，命名为菌株SFD31T。细胞革兰氏染色阴性，过氧化氢酶和氧化酶阳性。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株SFD31T在维诺格拉德斯基氏菌属内形成一个独立分支。菌株SFD31T与棘维诺格拉德斯基氏菌KMM 6211T（97.9%，16S rRNA基因序列相似性）、滨岸维诺格拉德斯基氏菌KMM 6491T（97.4%）、美丽维诺格拉德斯基氏菌EM106T（97.2%）和昆布维诺格拉德斯基氏菌EC29T（96.9%）的亲缘关系较远。菌株SFD31T的主要脂肪酸为异C15 : 0、异C15 : 1 G、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）以及未知脂肪酸13.565。该分离株唯一的类异戊二烯醌为甲基萘醌6。主要极性脂为磷脂酰乙醇胺、四种未鉴定的氨基脂和两种未鉴定的脂。菌株SFD31T的DNA G+C含量为36.0 mol%。表型特征将菌株SFD31T与维诺格拉德斯基氏菌属的相关种区分开来。基于本研究提供的证据，为菌株SFD31T（=KCTC 52348T=JCM 31798T）提出一个新物种，即黄色维诺格拉德斯基氏菌（Winogradskyella flava sp. nov.）。
