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采用高效液相色谱-紫外二极管阵列检测器和离子阱/飞行时间质谱检测法对虎杖根茎和根的成分进行分析。

Profiling of components of rhizoma et radix polygoni cuspidati by high-performance liquid chromatography with ultraviolet diode-array detector and ion trap/time-of-flight mass spectrometric detection.

作者信息

Fu Jinfeng, Wang Min, Guo Huimin, Tian Yuan, Zhang Zunjian, Song Rui

机构信息

Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, China.

Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, China ; Department of Pharmaceutical Analysis, China Pharmaceutical University, Tongjia Lane 24, Nanjing 210009, China.

出版信息

Pharmacogn Mag. 2015 Jul-Sep;11(43):486-501. doi: 10.4103/0973-1296.160455.

DOI:10.4103/0973-1296.160455
PMID:26246723
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4522834/
Abstract

INTRODUCTION

Rhizoma et Radix Polygoni Cuspidati (Huzhang in Chinese, HZ) is a traditional medicinal plant in China. Many of the components of HZ have been proved to be bioactive while it is difficult to conduct a comprehensive chemical profiling of HZ as a consequence of the absence of efficient separation system and sensitive detective means. We developed a simple and effective method for comprehensive characterization of constituents in HZ.

OBJECTIVE

To develop a simple and effective method to characterize the components in HZ and provide useful information for subsequent metabolic studies of HZ.

MATERIALS AND METHODS

The components in HZ aqueous extract were characterized by using high performance liquid chromatography with UV diode-array detector (HPLC-DAD) and ion trap/time-of-flight mass spectrometric detection (HPLC-IT/TOF). Stilbenes, anthraquinones, gallates and tannins, naphthalenes and some other compounds were identified and confirmed by diagnostic fragment ions with accurate mass measurements, characteristic fragmentation pathways and relevant published literatures.

RESULTS

Among the 238 constituents detected in HZ, a total number of 74 constituents were identified unambiguously or tentatively, including 29 compounds reported for the first time in HZ.

CONCLUSION

The identification and structure elucidation of these chemicals provided essential data for quality control and further in vivo metabolic studies of HZ. Key words: Polygonum cuspidatum, HPLC-DAD, HPLC-IT/TOF, qualitative analysis.

摘要

引言

虎杖是中国的一种传统药用植物。虎杖的许多成分已被证明具有生物活性，但由于缺乏高效的分离系统和灵敏的检测手段，对其进行全面的化学剖析较为困难。我们开发了一种简单有效的方法来全面表征虎杖中的成分。

目的

开发一种简单有效的方法来表征虎杖中的成分，并为虎杖后续的代谢研究提供有用信息。

材料与方法

采用配有紫外二极管阵列检测器的高效液相色谱法（HPLC-DAD）和离子阱/飞行时间质谱检测法（HPLC-IT/TOF）对虎杖水提取物中的成分进行表征。通过具有精确质量测量的诊断性碎片离子、特征性裂解途径及相关已发表文献对芪类、蒽醌类、没食子酸盐和单宁类、萘类及其他一些化合物进行鉴定和确认。

结果

在虎杖中检测到的238种成分中，共明确或初步鉴定出74种成分，其中有29种化合物为首次在虎杖中报道。

结论

这些化学成分的鉴定和结构解析为虎杖的质量控制及进一步的体内代谢研究提供了重要数据。关键词：虎杖；HPLC-DAD；HPLC-IT/TOF；定性分析

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8239/4522834/0e533a5a7b29/PM-11-486-g016.jpg
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