鳞叶冷银柴提取物具有体外清除自由基、抗氧化潜力以及对离子介导的肝酶氧化损伤的保护特性。

Afrostyrax lepidophyllus extracts exhibit in vitro free radical scavenging, antioxidant potential and protective properties against liver enzymes ion mediated oxidative damage.

作者信息

Moukette Bruno Moukette, Pieme Constant Anatole, Biapa Prosper Cabral Nya, Moor Vicky Jocelyne Ama, Berinyuy Eustace, Ngogang Jeanne Yonkeu

机构信息

Laboratory of Biochemistry, Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon.

Department of Biochemistry, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon.

出版信息

BMC Res Notes. 2015 Aug 12;8:344. doi: 10.1186/s13104-015-1304-8.

DOI:10.1186/s13104-015-1304-8

PMID:26264271

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4534151/

Abstract

BACKGROUND

Several studies described the phytochemical constituents of plants in relation with the free radical scavenging property and inhibition of lipid peroxidation. This study investigated the in vitro antioxidant property, and the protective effects of ethanolic and aqueous ethanol extract of the leaves and barks of Afrostyrax lepidophyllus (Huaceae) against ion mediated oxidative damages.

METHODS

Four extracts (ethanol and aqueous-ethanol) from the leaves and barks of A. lepidophyllus were used in this study. The total phenols content, the antiradical and antioxidant properties were determined using standard colorimetric methods.

RESULTS

The plant extracts had a significant scavenging potential on the 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), nitrite oxide (NO) and 2,2-azinobis (3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals with the IC50 varied between 47 and 200 µg/mL depending on the part of plant and the type of extract. The ethanol extract of A. lepidophyllus bark (GEE) showed the highest polyphenolic (35.33 ± 0.29) and flavonoid (12.00 ± 0.14) content. All the tested extracts demonstrated a high protective potential with the increased of superoxide dismutase, catalase and peroxidase activities.

CONCLUSION

Afrostyrax lepidophyllus extracts exhibited higher antioxidant potential and significant protective potential on liver enzymes.

摘要

背景

多项研究描述了植物的植物化学成分与自由基清除特性及脂质过氧化抑制之间的关系。本研究调查了鳞叶冷杉（胡桃科）叶和树皮的乙醇提取物及水乙醇提取物的体外抗氧化特性及其对离子介导的氧化损伤的保护作用。

方法

本研究使用了鳞叶冷杉叶和树皮的四种提取物（乙醇提取物和水乙醇提取物）。采用标准比色法测定总酚含量、抗自由基和抗氧化特性。

结果

植物提取物对2,2-二苯基-1-苦基肼（DPPH）、羟基（OH）、一氧化氮（NO）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基具有显著的清除潜力，IC50值在47至200μg/mL之间，具体取决于植物部位和提取物类型。鳞叶冷杉树皮的乙醇提取物（GEE）显示出最高的多酚含量（35.33±0.29）和黄酮含量（12.00±0.14）。所有测试提取物均表现出较高的保护潜力，超氧化物歧化酶、过氧化氢酶和过氧化物酶活性均有所增加。