调控DNA的功能足迹分析
Functional footprinting of regulatory DNA.
作者信息
Vierstra Jeff, Reik Andreas, Chang Kai-Hsin, Stehling-Sun Sandra, Zhou Yuanyue, Hinkley Sarah J, Paschon David E, Zhang Lei, Psatha Nikoletta, Bendana Yuri R, O'Neil Colleen M, Song Alexander H, Mich Andrea K, Liu Pei-Qi, Lee Gary, Bauer Daniel E, Holmes Michael C, Orkin Stuart H, Papayannopoulou Thalia, Stamatoyannopoulos George, Rebar Edward J, Gregory Philip D, Urnov Fyodor D, Stamatoyannopoulos John A
机构信息
Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
Sangamo BioSciences, Pt. Richmond, California, USA.
出版信息
Nat Methods. 2015 Oct;12(10):927-30. doi: 10.1038/nmeth.3554. Epub 2015 Aug 31.
Regulatory regions harbor multiple transcription factor (TF) recognition sites; however, the contribution of individual sites to regulatory function remains challenging to define. We describe an approach that exploits the error-prone nature of genome editing-induced double-strand break repair to map functional elements within regulatory DNA at nucleotide resolution. We demonstrate the approach on a human erythroid enhancer, revealing single TF recognition sites that gate the majority of downstream regulatory function.
调控区域含有多个转录因子（TF）识别位点；然而，单个位点对调控功能的贡献仍难以确定。我们描述了一种利用基因组编辑诱导的双链断裂修复的易错性质，以核苷酸分辨率绘制调控DNA内功能元件图谱的方法。我们在人类红系增强子上展示了该方法，揭示了控制大部分下游调控功能的单个TF识别位点。
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