Lei Na, Wang Mi, Zhang Lifang, Xiao Sui, Fei Chengzhong, Wang Xiaoyang, Zhang Keyu, Zheng Wenli, Wang Chunmei, Yang Ruile, Xue Feiqun
Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Int J Mol Sci. 2015 Sep 8;16(9):21575-90. doi: 10.3390/ijms160921575.
To evaluate the antioxidant and immune effects of low molecular yeast β-glucan on mice, three sulfated glucans from Saccharomyces cerevisiae (sGSCs) with different molecular weight (MW) and degrees of sulfation (DS) were prepared. The structures of the sGSCs were analyzed through high performance liquid chromatography-gel permeation chromatography (HPLC-GPC) and Fourier transform infrared spectroscopy (FTIR). sGSC1, sGSC2, and sGSC3 had MW of 12.9, 16.5 and 19.2 kDa, respectively, and DS of 0.16, 0.24 and 0.27, respectively. In vitro and in vivo experiments were conducted to evaluate the antioxidant and immunological activities of the sGSCs. In vitro experiment, the reactive oxygen species (ROS) scavenging activities were determined. In vivo experiment, 50 male BALB/c mice were divided into five groups. The sGSC1, sGSC2 and sGSC3 treatment groups received the corresponding sGSCs at 50 mg/kg/day each. The GSC (glucans from Saccharomyces cerevisiae) treatment group received 50 mg/kg/day GSC. The normal control group received equal volume of physiological saline solution. All treatments were administered intragastrically for 14 day. Results showed that sGSC1, sGSC2 and sGSC3 can scavenge 1,1-diphenyl-2-picryl-hydrazyl (DPPH), superoxide, and hydroxyl radicals in vitro. The strength of the radical scavenging effects of the sGSCs was in the order of sGSC1 > sGSC2 > sGSC3. Oral administration of sGSC1 significantly improved serum catalase (CAT) and glutathione peroxidase (GSH-Px) activities and decreased malondialdehyde (MDA) level in mice. sGSC1 significantly improved the spleen and thymus indexes and the lymphocyte proliferation, effectively enhanced the percentage of CD4⁺ T cells, decreased the percentage of CD8⁺ T cells, and elevated the CD4⁺/CD8⁺ ratio. sGSC1 significantly promoted the secretion of IL-2 and IFN-γ. These results indicate that sGSC1 with low MW and DS has better antioxidant and immunological activities than the other sGSCs, and sGSC1 could be used as a new antioxidant and immune-enhancing agent.
为评估低分子酵母β-葡聚糖对小鼠的抗氧化和免疫作用，制备了三种来自酿酒酵母的硫酸化葡聚糖（sGSCs），它们具有不同的分子量（MW）和硫酸化程度（DS）。通过高效液相色谱-凝胶渗透色谱（HPLC-GPC）和傅里叶变换红外光谱（FTIR）分析了sGSCs的结构。sGSC1、sGSC2和sGSC3的分子量分别为12.9、16.5和19.2 kDa，硫酸化程度分别为0.16、0.24和0.27。进行了体外和体内实验以评估sGSCs的抗氧化和免疫活性。体外实验测定了活性氧（ROS）清除活性。体内实验中，将50只雄性BALB/c小鼠分为五组。sGSC1、sGSC2和sGSC3治疗组分别以50 mg/kg/天的剂量给予相应的sGSCs。GSC（来自酿酒酵母的葡聚糖）治疗组给予50 mg/kg/天的GSC。正常对照组给予等量的生理盐水溶液。所有处理均通过灌胃给药14天。结果表明，sGSC1、sGSC2和sGSC3在体外可清除1,1-二苯基-2-苦基肼（DPPH）、超氧阴离子和羟基自由基。sGSCs的自由基清除作用强度顺序为sGSC1>sGSC2>sGSC3。口服sGSC1可显著提高小鼠血清过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性，并降低丙二醛（MDA）水平。sGSC1可显著提高脾脏和胸腺指数以及淋巴细胞增殖，有效提高CD4⁺T细胞百分比，降低CD8⁺T细胞百分比，并提高CD4⁺/CD8⁺比值。sGSC1可显著促进IL-2和IFN-γ的分泌。这些结果表明，具有低分子量和硫酸化程度的sGSC1比其他sGSCs具有更好的抗氧化和免疫活性，且sGSC1可作为一种新型的抗氧化和免疫增强剂。
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