Zeng Huawei, Grapov Dmitry, Jackson Matthew I, Fahrmann Johannes, Fiehn Oliver, Combs Gerald F
Grand Forks Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Grand Forks, ND 58203, USA.
West Coast Metabolomics Center, University of California, Davis, CA 95616, USA.
Metabolites. 2015 Sep 11;5(3):489-501. doi: 10.3390/metabo5030489.
The pattern of metabolites produced by the gut microbiome comprises a phenotype indicative of the means by which that microbiome affects the gut. We characterized that phenotype in mice by conducting metabolomic analyses of the colonic-cecal contents, comparing that to the metabolite patterns of feces in order to determine the suitability of fecal specimens as proxies for assessing the metabolic impact of the gut microbiome. We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF). Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism. A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces. These data comprise the first characterization of relationships among metabolites present in the colonic-cecal contents and feces in a healthy mouse model, and shows that feces can be a useful proxy for assessing the pattern of metabolites to which the colonic mucosum is exposed.
肠道微生物群产生的代谢物模式构成了一种表型，该表型指示了该微生物群影响肠道的方式。我们通过对结肠-盲肠内容物进行代谢组学分析来表征小鼠的这种表型，并将其与粪便的代谢物模式进行比较，以确定粪便标本作为评估肠道微生物群代谢影响的替代物的适用性。我们通过气相色谱-飞行时间质谱（GC-TOF）和超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF）在结肠-盲肠内容物和粪便中总共检测到270种低分子量代谢物。其中，251种（93%）在两种类型的标本中都存在，几乎代表了所有与氨基酸、碳水化合物、能量、脂质、膜转运、核苷酸、遗传信息处理和癌症相关代谢有关的已知生化途径。结肠-盲肠内容物和粪便之间共有115种代谢物的相对丰度存在显著差异。这些数据首次表征了健康小鼠模型中结肠-盲肠内容物和粪便中存在的代谢物之间的关系，并表明粪便可以作为评估结肠黏膜所接触的代谢物模式的有用替代物。
