Okagaki Laura H, Nunes Cristiano C, Sailsbery Joshua, Clay Brent, Brown Doug, John Titus, Oh Yeonyee, Young Nelson, Fitzgerald Michael, Haas Brian J, Zeng Qiandong, Young Sarah, Adiconis Xian, Fan Lin, Levin Joshua Z, Mitchell Thomas K, Okubara Patricia A, Farman Mark L, Kohn Linda M, Birren Bruce, Ma Li-Jun, Dean Ralph A
Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606.
Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606.
G3 (Bethesda). 2015 Sep 28;5(12):2539-45. doi: 10.1534/g3.115.020057.
Magnaporthaceae is a family of ascomycetes that includes three fungi of great economic importance: Magnaporthe oryzae, Gaeumannomyces graminis var. tritici, and Magnaporthe poae. These three fungi cause widespread disease and loss in cereal and grass crops, including rice blast disease (M. oryzae), take-all disease in wheat and other grasses (G. graminis), and summer patch disease in turf grasses (M. poae). Here, we present the finished genome sequence for M. oryzae and draft sequences for M. poae and G. graminis var. tritici. We used multiple technologies to sequence and annotate the genomes of M. oryzae, M. poae, and G. graminis var. tritici. The M. oryzae genome is now finished to seven chromosomes whereas M. poae and G. graminis var. tritici are sequenced to 40.0× and 25.0× coverage respectively. Gene models were developed by the use of multiple computational techniques and further supported by RNAseq data. In addition, we performed preliminary analysis of genome architecture and repetitive element DNA.
稻瘟病菌科是子囊菌的一个科，其中包括三种具有重大经济意义的真菌：稻瘟病菌、小麦全蚀病菌小麦变种和早熟禾叶斑病菌。这三种真菌在谷类和禾本科作物中引发广泛病害并造成损失，包括稻瘟病（由稻瘟病菌引起）、小麦和其他禾本科植物的全蚀病（由小麦全蚀病菌引起）以及草坪草的夏季斑枯病（由早熟禾叶斑病菌引起）。在此，我们展示了稻瘟病菌的完整基因组序列以及早熟禾叶斑病菌和小麦全蚀病菌小麦变种的草图序列。我们使用了多种技术对稻瘟病菌、早熟禾叶斑病菌和小麦全蚀病菌小麦变种的基因组进行测序和注释。稻瘟病菌的基因组现已完成至七条染色体，而早熟禾叶斑病菌和小麦全蚀病菌小麦变种的测序覆盖度分别为40.0倍和25.0倍。通过使用多种计算技术构建了基因模型，并得到了RNA测序数据的进一步支持。此外，我们对基因组结构和重复元件DNA进行了初步分析。
